Mass spectrometry based proteomics,background,status and future needs  

作　　者：Peter Roepstorff 

机构地区：[1]Department of Biochemistry and Molecular Biology,University of Southern Denmark Campusvej 55,DK 5230 Odense M,Denmark

出　　处：《Protein & Cell》2012年第9期641-647,共7页蛋白质与细胞（英文版）

摘　　要：An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in proteomics.The advantages and limitations of the two major strategies,2D-gel based and LC-MS based,are discussed and a combination for the two,CeLC-MS is described.A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are described.Finally the present status and future needs discussed.

关 键 词：PROTEOMICS 2D-PAGE LC-MS ISOFORMS PHOSPHORYLATION 

分 类 号：O62[理学—有机化学]