Microvesicle-delivery miR-150 promotes tumorigenesis by up-regulating VEGF,and the neutralization of miR-150 attenuate tumor development  

作　　者：Yuchen Liu Luming Zhao Dameng Li Yuan Yin Chen-Yu Zhang Jing Li Yujing Zhang 

机构地区：[1]Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology,State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210093,China [2]Oncology Institute,Fourth Affiliated Hospital of Suzhou University,Wuxi 214062,China

出　　处：《Protein & Cell》2013年第12期932-941,共10页蛋白质与细胞（英文版）

基　　金：This work was supported by grants from the National Basic Research Program(973 Program)(No.2014CB542300);the National Natural Science Foundation of China(Grant Nos.31200969,31200874,81101330,31271378,and 81250044);the National Science Fund for Distinguished Young Scholars(81025019);the Natural Science Foun-dation of Jiangsu Province(BK2011013 and BK2012014);the Research Fund for the Doctoral Program of Higher Education of China(20120091120044).

摘　　要：Tumor-associated macrophages(TAMs)mostly exhibit M2-like(alternatively activated)properties and play posi-tive roles in angiogenesis and tumorigenesis.Vascular en-dothelial growth factor(VEGF)is a key angiogenic factor.During tumor development,TAMs secrete VEGF and other factors to promote angiogenesis;thus,anti-treatment against TAMs and VEGF can repress cancer development,which has been demonstrated in clinical trials and on an experimental level.In the present work,we show that miR-150 is an oncomir because of its promotional effect on VEGF.MiR-150 targets TAMs to up-regulate their secre-tion of VEGF in vitro.With the utilization of cell-derived vesicles,named microvesicles(MVs),we transferred an-tisense RNA targeted to miR-150 into mice and found that the neutralization of miR-150 down-regulates miR-150 and VEGF levels in vivo and attenuates angiogenesis.There-fore,we proposed the therapeutic potential of neutralizing miR-150 to treat cancer and demonstrated a novel,natu-ral,microvesicle-based method for the transfer of nucleic acids.

关 键 词：MICROVESICLE miR-150 TUMORIGENESIS VEGF NEUTRALIZATION ATTENUATION 

分 类 号：R73[医药卫生—肿瘤]