乌饭树树叶蓝黑色素对α-葡萄糖苷酶的抑制活性研究  

Study on theα-glucosidase inhibition of the dark blue pigment from the leaves of Vaccinium bracteatum Thunb.

作　　者：包正宇 樊铭聪 李言[1,2] 钱海峰 张晖[1,2] 齐希光 王立[1,2] BAO Zheng-yu;FAN Ming-cong;LI Yan;QIAN Hai-feng;ZHANG Hui;QI Xi-guang;WANG Li(School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]食品科学与工程国家重点实验室,江苏无锡214122

出　　处：《食品与机械》2021年第7期165-170,176,共7页Food and Machinery

基　　金：国家自然科学基金资助项目(编号:31671890)。

摘　　要：目的:研究乌饭树树叶蓝黑色素对α-葡萄糖苷酶活性的影响以验证其对调节餐后血糖的有益作用。方法:通过富集乌饭树树叶中环烯醚萜苷化合物与外源游离氨基化合物反应制备蓝黑色素,探究其对α-葡萄糖苷酶活性的影响,采用Lineweaver-Burk双倒数曲线法和荧光发射光谱法分析其抑制动力学及其相互作用。结果:乌饭树树叶蓝黑色素对α-葡萄糖苷酶活性具有较好的抑制作用,抑制活性随色素浓度的增加逐渐升高,IC_(50)值为0.373 mg/mL。抑制动力学研究表明蓝黑色素属于竞争性和非竞争性的混合抑制类型,荧光光谱结果分析表明色素可导致α-葡萄糖苷酶分子结构解折叠而使其失活。结论:乌饭树树叶蓝黑色素表现出的α-葡萄糖苷酶抑制活性可辅助调节餐后血糖。Objective:Theα-glucosidase inhibition(α-GI)activity of dark blue pigment from the leaves of Vaccinium bracteatum Thunb.(VBTL)was investigated to confirm the benefits of regulation of postprandial blood sugar.Methods:The dark blue pigment prepared by purified iridoid compounds and exogenous amino compound was used to study the inhibition effect and the inhibition kinetic by Lineweaver-Burk reciprocal curve method.Fluorescence emission spectrum was used to analyze the interaction between the pigment andα-glucosidase.Results:The VBTL dark blue pigment had higher inhibitory effect onα-glucosidase.Theα-GI activity increased with the increasing concentration of dark blue pigment and the IC_(50) value was 0.373 mg/mL.The inhibition kinetic analysis showed that theα-glucosidase inhibitory effect of dark blue pigment was competitive and non-competitive mixed inhibition types.Fluorescence spectrum analysis showed that the static quenching model was happened betweenα-glucosidase and pigment molecules,and this interaction caused the unfolding of theα-glucosidase molecule structure.Conclusion:Theα-GI activity of dark blue pigment from VBTL was verified its beneficial effect on regulating postprandial blood glucose,and provided the scientific basis for the development and utilization of pigment of VBTL.

关 键 词：乌饭树树叶 蓝黑色素 Α-葡萄糖苷酶 抑制活性 

分 类 号：R284[医药卫生—中药学]