超声波辅助高压法提取金针菇黄酮的纯化及活性测定  

Purification and Activity Determination of Flavonoids from Flammulina Velutipes Extracted by Ultrasonic Assisted High Pressure Method

作　　者：王广慧[1] 于德涵[1] 黎莉[1] WANG Guanghui;YU Dehan;LI Li(College of Food and Pharmaceutical Engineering,Suihua University^Suihua 152061,China)

机构地区：[1]绥化学院食品与制药工程学院,黑龙江绥化152061

出　　处：《东北农业科学》2021年第3期100-105,共6页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省绥化市科技计划项目(SHKJ2017-075);国家自然科学基金项目(21201128)。

摘　　要：为了获知超声波辅助高压法所提取金针菇黄酮的抑菌和抗氧化活性以及D101型大孔吸附树脂纯化金针菇黄酮的最优条件,利用D101型大孔树脂对超声波辅助高压法所提取的金针菇黄酮进行纯化,并对纯化条件进行了优化研究,而后对纯化后黄酮抑制大肠杆菌、枯草芽孢杆菌、沙门氏菌和金黄色葡萄球菌的能力进行了检测;又以抗坏血酸(VC)为对照,对黄酮清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、超氧阴离子自由基(·O_(2)^(-))、羟基自由基(·OH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS·)的能力进行测定。结果表明,D101型大孔树脂纯化金针菇黄酮的最适条件为:采用浓度为1.2 mg/m L、p H值为5的上样液,在上样流速为0.75 m L/min的条件下进行吸附;吸附饱和后用70%的乙醇60 m L以1 m L/min洗脱。在此条件下得到的黄酮纯度是纯化前的2.71倍。超声波辅助高压法所提取的金针菇黄酮对四种菌均有不同程度的抑制作用,其抑菌程度大小顺序为:大肠杆菌>沙门氏菌>金黄色葡萄球菌>枯草芽孢杆菌。金针菇黄酮对DPPH·、·O_(2)^(-)、·OH和ABTS·均具有一定的清除能力,但弱于VC。可知超声波辅助高压法所提取的金针菇黄酮具有抑菌及抗氧化活性,D101型大孔吸附树脂纯化金针菇黄酮效果较好。In order to obtain the antibacterial and antioxidative activities of Flammulina flavonoids extracted by ul-trasonic assisted high pressure method and the optimal conditions for purification by D101 macroporous resin,theresin was used to purify the flavonoids extracted by ultrasonic assisted high pressure method in the paper.The purifi-cation conditions were optimized,and the ability of the purified flavonoids to inhibit Escherichia coli,Bacillus subtilis,Salmonella enteritidis and Staphylococcus aureus was detected.The ability of flavonoids to remove DPPH·,·O_(2)^(-),·OHand 2,2-binitro-bis ABTS·was determined with Vc as a comparison.The results showed that the optimum condi-tions for the purification were as follows:the adsorption solution was prepared at a concentration of 1.2 mg/m L and ap H of 5,and the adsorption was carried out at a loading flow rate of 0.75 m L/min.After saturation,it was eluted with70% ethanol 60 m L at 1 m L/min.The purity of the flavonoids obtained under these conditions was 2.71 times thatbefore purification.The Flammulina flavonoids extracted by ultrasonic assisted high pressure method have differentinhibitory effects on the four strains.The order of inhibition is:Escherichia coli>Salmonella enteritidis>Staphylococcus aureus>Bacillus subtilis.The Flammulina flavonoids have certain scavenging ability to DPPH·,·O_(2)^(-),·OH and ABTS·,but weaker than Vc.It can be seen that the Flammulina flavonoids extracted by the method have antibacteri-al and antioxidative activities,and D101 macroporous adsorption resin has better effect on purifying Flammulina fla-vonoids.

关 键 词：超声波辅助高压法 金针菇黄酮 大孔吸附树脂 抑菌性 抗氧化性 

分 类 号：TS201.2[轻工技术与工程—食品科学]