膜分离工艺提高产品中高聚合度ε-聚赖氨酸含量  

Increasing theε-PL content with high degree of polymerization in products using membrane separation

作　　者：赵星宇 陈旭升[1,2] 张宏建[1,2] 张建华[1,2] ZHAO Xingyu;CHEN Xusheng;ZHANG Hongjian;ZHANG Jianhua(School of Biotechnology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,江苏无锡214122 [2]江南大学,工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2021年第14期196-201,共6页Food and Fermentation Industries

基　　金：江苏省产学研联合创新项目(BY2016022-25);江苏省自然科学基金面上项目(BK20191332)。

摘　　要：为了提高产品中高聚合度ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)的含量,作者在膜筛选基础上,以模拟料液为研究对象,通过单因素实验优化了膜分离工艺参数,比对了分离前后产品的抑菌性能,并分析了不同聚合度ε-PL透过膜的方式。结果表明,截留分子质量为3000 Da的聚醚砜(polyethersulfone,PES)膜最适合用于筛分聚合度25前后的ε-PL,操作压力为0.25 MPa,pH=6,质量浓度为20 g/L的料液经透析后,循环液中聚合度25~35的ε-PL含量提高至91.22%,透析后的产品更有利于抑制金黄色葡萄球菌和大肠杆菌。膜分离工艺不能实现不同聚合度ε-PL的绝对分离,但是利用不同聚合度ε-PL透过膜的速率不同,可以提高产品中高聚合度ε-PL的比例,从而提高产品的抑菌性能,对于提高ε-PL应用价值具有重要意义。To increase the content ofε-poly-L-lysine(ε-PL)with high degree of polymerization,membrane was screened and the membrane separation process parameters were optimized.The antibacterial properties of the products before and after separation were compared,while the wayε-PL penetrated the membrane was also analyzed.Results showed that the polyethersulfone(PES)membrane with a molecular weight cut-off of 3000 Da was the best.At 0.25 MPa,pH 6,and 20 g/L feed solution,the content ofε-PL with a degree of polymerization of 25-35 was increased to 91.22%,and the product after filtering was more conducive to inhibit Staphylococcus aureus and Escherichia coli.The membrane was not able to achieve absolute separation ofε-PL,but it increased the proportion ofε-PL with high polymerization degrees and improved the antibacterial performance.The study is of great significance to improve the application value ofε-PL.

关 键 词：Ε-聚赖氨酸 防腐剂 聚合度 膜分离 最小抑菌浓度 

分 类 号：TS202.3[轻工技术与工程—食品科学] TQ028.8[轻工技术与工程—食品科学与工程]