响应面法优化酶解制备鱼皮胶原寡肽  

Enzymolysis Preparation of Fish Skin Collagen Peptide by Response Surface Test

作　　者：王霞[1] 毕桂灿[2] 赖园园 WANG Xia;BI Guican;LAI Yuanyuan(Institute of Biotechnology,Guangdong Food and Drug Vocational College,Guangzhou 510000,China;Institute of Biomass Engineering,South China Agricultural University,Guangzhou 510000,China;Anhui Guotai Biotechnology Co.,Ltd.,Xuancheng 242000,China)

机构地区：[1]广东食品药品职业学院生物技术学院,广东广州510000 [2]华南农业大学新能源与新材料研究所,广东广州510000 [3]安徽国肽生物科技有限公司,安徽宣城242000

出　　处：《现代食品》2021年第10期62-66,共5页Modern Food

基　　金：广东食品药品职业学院自然科学研究一般项目(2019ZR16)。

摘　　要：以鱼皮为原料,SCAU胶原蛋白复合酶水解制备胶原寡肽。在单因素分析基础上,结合响应面法,考查加酶量、温度、时间、pH值4个因素对胶原寡肽提取率的影响。结果显示,最佳酶解条件为:每克鱼皮(干)加酶0.305 mL、时间4.2 h、温度54.6℃、pH值8.2。在此条件下进行3次平行试验,鱼皮胶原寡肽平均提取率为22.58%。方差分析表明,各因素对鱼皮胶原寡肽提取率影响为pH值>时间>温度>加酶量。The collagen peptide was prepared by SCAU collagen complex enzyme which was hydrolyzed from fish skin. On a single factor basis, combined with response surface test, investigate the effect of various factors on the extraction rate of collagen peptide according to the factors of enzyme amount, hydrolysis temperature, hydrolysis time and pH. The results showed that optimum enzymatic hydrolysis conditions were enzyme amount 0.305 mL·g^(-1) fish skin(dry), hydrolysis time 4.2 h, hydrolysis temperature 54.6 ℃, pH 8.2. 3 times parallel test under this process parameter, the extraction rate was 22.58%. The results of variance analysis showed that the factors affecting the extraction rate of collagen peptide were pH >hydrolysis time >hydrolysis temperature >enzyme content.

关 键 词：胶原肽 响应面 酶解 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]