元宝枫籽多肽的制备及其抗氧化性的研究  

Study on Preparation and Antioxidant Activity of Polypeptides from the Seeds of Acer truncatum

作　　者：刘速速 李文凯 孙华 周庆礼[1] 于功明 马强 LIU Su-su;LI Wen-kai;SUN Hua;ZHOU Qing-li;YU Gong-ming;MA Qiang(College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China;Food Science and Engineering,Qilu University of Technology,Jinan 250353,China;College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457 [2]齐鲁工业大学食品科学与工程学院,济南250353 [3]上海海洋大学食品学院,上海201306

出　　处：《中国调味品》2021年第8期6-11,共6页China Condiment

摘　　要：通过单因素试验对胰蛋白酶水解条件进行筛选,并采用正交试验优化酶解条件,得到最终酶解条件为:底物浓度2%、酶解时间5 h、加酶量12000 U/g、pH 7.5,此时DPPH清除率为46.2%。再将多肽溶液进行超滤分离,得到不同分子质量的水解产物,收集产物并进行冷冻干燥,测定不同分子量肽的抗氧化活性。分子量<3 kDa的抗氧化活性最高,达到70.4%。对活性最高的组分(<3 kDa)进行稳定性的研究,最终得到元宝枫籽抗氧化肽在20~60℃、pH为7、NaCl为0.2%或0.5%、葡萄糖为1%~2%的条件下抗氧化肽较为稳定,DPPH清除率基本不变。The hydrolysis conditions of trypsin are screened by single factor test,and the enzymatic hydrolysis conditions are optimized by orthogonal test.The final enzymatic hydrolysis conditions are as follows:substrate concentration is 2%,enzymatic hydrolysis time is 5 h,enzyme dosage is 12000 U/g,pH is 7.5,and DPPH clearance rate is 46.2%.And then the polypeptide solution is separated by ultrafiltration to obtain hydrolysates with different molecular weights.The hydrolysates are collected and freeze-dried to determine the antioxidant activity of peptides with different molecular weights.The peptide with molecular weight<3 kDa has the highest antioxidant activity of 70.4%.The stability of the most active component(<3 kDa)is studied.Finally,the antioxidative peptides are relatively stable under the conditions of 20~60℃,pH 7,NaCl 0.2% or 0.5%,glucose 1%~2%,and DPPH clearance rate is basically unchanged.

关 键 词：元宝枫籽蛋白 抗氧化肽 胰蛋白酶 稳定性 

分 类 号：TS202.3[轻工技术与工程—食品科学]