An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo  

作　　者：Wenjing Wei Yilin Song Li Wang Song Zhang Jinping Luo Shengwei Xu Xinxia Cai 

机构地区：[1]State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Microsystems & Nanoengineering》2015年第1期159-164,共6页微系统与纳米工程（英文）

基　　金：This work was sponsored by the Major National Scientific Research Plan(Grant Nos.2011CB933202 and 2014CB744605);the NSFC(Grant Nos.61125105 and 61471342);the Beijing Science and Technology Plan(Grant Nos.Z141100000214002 and Z141102003414014);the Key Programs of the Chinese Academy of Sciences(Grant No.KJZD-EW-L11-2).

摘　　要：L-glutamate,the most common excitatory neurotransmitter in the mammalian central nervous system(CNS),is associated with a wide range of neurological diseases.Because neurons in CNS communicate with each other both electrically and chemically,dualmode(electric and chemical)analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in vivo.In the present study,a silicon-based implantable microelectrode array(MEA)composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical system.In the MEA probe,the electrophysiological electrodes have a low impedance of 0.018 MΩat 1 kHz,and the electrochemical electrodes show a sensitivity of 56 pAμM^(−1) to glutamate and have a detection limit of 0.5μM.The MEA probe was used to monitor extracellular glutamate levels,spikes and local field potentials(LFPs)in the striatum of anaesthetised rats.To explore the potential of the MEA probe,the rats were administered to KCl via intraperitoneal injection.K+significantly increases extracellular glutamate levels,LFP low-beta range(12–18 Hz)power and spike firing rates with a similar temporal profile,indicating that the MEA probe is capable of detecting dual-mode neuronal signals.It was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo.

关 键 词：MEMS implantable microelectrode array GLUTAMATE electrophysiological detection in vivo 

分 类 号：R73[医药卫生—肿瘤]