1株纳豆激酶高产菌株的分离·筛选与复合诱变研究  

Isolation, Screening and Compound Mutagenesis of a Nattokinase Producing Strain

作　　者：巩涛[1] 魏传军 安明理[1] 宁萌[1] 周伏忠[1] GONG Tao;WEI Chuan-jun;AN Ming-li(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou,Henan 450008)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008

出　　处：《安徽农业科学》2021年第15期159-161,175,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省科技攻关项目(212102310541);河南省科学院科研项目(190305003、18JK16012)。

摘　　要：为选育纳豆激酶(Nattokinase,NK)高活性菌株,从4种不同风味食品中分离得到26株菌株,通过酪蛋白平板初筛和液体发酵复筛,得到2株纳豆激酶高产菌株,利用分子生物学技术鉴定为2株枯草芽孢杆菌(Bacillus subtilis)。采用氯化锂-紫外与亚硝基胍(NTG)复合诱变的方式,最终获得1株纳豆激酶活性达到2338.01 U/mL的高产菌株SD3-3-6,该突变菌株纳豆激酶酶活相较于初始菌株SD3提高了1.93倍,是1株具有应用前景的高产菌株。In order to select nattokinase high activity strains,26 strains were isolated from four different samples.Two nattokinase high-yield strains were screened through primary screening and rescreening,and identified as two Bacillus subtilis strains by molecular biology technology.A high-yield strain SD3-3-6 with nattokinase activity of 2338.01 U/mL was obtained by lithium chloride UV and NTG compound mutagenesis.The final activity of nattokinase of SD3-3-6 was 1.93 times higher than that of the original strain SD3.

关 键 词：纳豆 纳豆激酶 多肽 复合诱变 芽孢杆菌 

分 类 号：Q936.96[生物学—微生物学]