响应面优化亚临界水提取茶色素工艺研究  

Study on Response Surface Optimization for Extraction of Tea Pigment from Subcritical Water

作　　者：刘丽辰 黄秀红 阮怿航 张禄焕 赵璇 林金科[2] LIU Li-chen;HUANG Xiu-hong;RUAN Yi-hang;ZHANG Lu-huan;ZHAO Xuan;LIN Jin-ke(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Anxi College of Tea Science,Fujian Agriculture and Forestry University,Quanzhou 362400,Fujian,China)

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建农林大学安溪茶学院,福建泉州362400

出　　处：《食品研究与开发》2021年第14期71-79,共9页Food Research and Development

基　　金：福建省高校产学合作项目(2016N5001);保健茶及细微茶粉研究(KF2015122);福建省安溪县现代农业产业园协同创新中心项目(KMD18003A)。

摘　　要：该研究利用响应面法优化亚临界水提取茶色素工艺。考察料液比、提取温度、提取时间对茶色素提取效果的影响。经响应面试验优化后,确定亚临界水最佳提取条件为料液比1∶50(g/m L),提取温度138.1℃,提取时间9.87 min。提取得到的茶黄素、茶红素、茶褐素、总茶色素含量分别为1.31%、14.37%、13.34%、29.02%,且具有良好的抗氧化活性。用电镜扫描观察提取后的总茶色素物理形态变化,发现其结构疏松。In this study,the response surface method was used to optimize the extraction of tea pigment from subcritical water. The effects of solid-liquid ratio,extraction temperature and extraction time on extraction efficiency of tea pigment were investigated. After optimization of response surface experiment,the optimal extraction conditions of subcritical water were determined as follows:solid-liquid ratio 1∶50(g/mL),extraction temperature 138.1 ℃,extraction time 9.87 min. The maximum total contents of theaflavin,thearubigin,theabrownin and the total tea pigment that could be extracted were 1.31%,14.37%,13.34%and 29.02%,and it had good antioxidant activity. Scanning electron microscopy was used to observe the physical changes of tea after extraction,and it was found that the structure of tea powder was loose,which was conducive to the precipitation of natural products.

关 键 词：茶黄素 茶红素 茶褐素 亚临界水 响应面分析 电镜扫描 

分 类 号：TS264.4[轻工技术与工程—发酵工程]