暹罗芽孢杆菌LW-1产γ-聚谷氨酸发酵培养基的优化  

Optimization of Fermentation Medium forγ-Polyglutamic Acid Production by Bacillus siamese LW-1

作　　者：蔡亚慧 王青 王文玉 皇高峰 张继冉[1] 徐淑霞[1] 张世敏[1] 吴坤[1] CAI Yahui;WANG Qing;WANG Wenyu;HUANG Gaofeng;ZHANG Jiran;XU Shuxia;ZHANG Shimin;WU Kun(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《食品工业科技》2021年第16期163-170,共8页Science and Technology of Food Industry

基　　金：河南省高等学校重点科研项目(19A180017)。

摘　　要：为提高暹罗芽孢杆菌LW-1(Bacillus Siamese LW-1)的γ-聚谷氨酸(γ-PGA)产量,基于单因素实验的结果,利用Plackett-Burman以及最陡爬坡实验确定响应面的最佳区域,设计一个三因素三水平的Box-Behnken实验来得到暹罗芽孢杆菌LW-1的最适培养基配方。结果表明,暹罗芽孢杆菌LW-1的最佳培养基配方为:谷氨酸钠86.71 g/L,柠檬酸钠17.94 g/L,MgSO_(4)·7H_(2)O 2.11 g/L,甘油25 g/L,KH_(2)PO_(4)1.4 g/L,(NH_(4))_(2)SO_(4)14 g/L,MnSO_(4)0.075 g/L,CaCl_(2)0.1 g/L,FeCl_(3)·6H_(2)O 0.04 g/L,在该培养基中γ-PGA产量达到44.78 g/L,与理论预测的最大值45.91 g/L非常接近,比未优化时(23.26 g/L)的γ-PGA产量提高了1.93倍。In order to increase the production ofγ-polyglutamic acid(γ-PGA)from Bacillus siamese LW-1,three significant factors affecting yield ofγ-PGA from the initial fermentation medium components were screened through the Plackett-Burman(PB)and the steepest climbing experiment based on the single-factor experiment to determine the optimal area of the response surface;the optimal medium components of Bacillus siamese LW-1 were obtained by using a three-factor and three-level Box-Behnken experiment.The optimal medium components for Bacillus siamese LW-1 producingγ-PGA were:sodium glutamate 86.71 g/L,sodium citrate 17.94 g/L,MgSO_(4)·7H_(2)O 2.11 g/L,glycerol 25 g/L,KH_(2)PO_(4)1.4 g/L,(NH_(4))_(2)SO_(4)14 g/L,MnSO_(4)0.075 g/L,CaCl_(2)0.1 g/L,FeCl_(3)·6H_(2)O 0.04 g/L.Under this optimum condition,the yield ofγ-PGA was 44.78 g/L which was 1.93 times that of before optimization(23.26 g/L),and it was very close to the maximum value 45.91 g/L predicted by the theory.

关 键 词：暹罗芽孢杆菌 Γ-聚谷氨酸 响应面法 PLACKETT-BURMAN 试验 发酵培养基 

分 类 号：TS201.1[轻工技术与工程—食品科学]