基于液相色谱-四极杆飞行时间质谱方法分析冰鲜鸡肉代谢标志物  

Analysis of Metabolic Markers in Chilled Chicken Based on Liquid Chromatography-Quadruple Time-of-Flight Mass Spectrometry

作　　者：王珂雯 徐雷 徐贞贞[1] 王雪[1] 杨曙明[1] WANG Kewen;XU Lei;XU Zhenzhen;WANG Xue;YANG Shuming(Key Laboratory of Agro-food Safety and Quality,Ministry of Agriculture and Rural Affairs,Institute of Quality Standard&Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,农业农村部农产品质量安全重点实验室,北京100081 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品科学》2021年第16期293-303,共11页Food Science

基　　金：2018年国家畜禽产品质量安全风险评估项目(GJFP201800704)。

摘　　要：采用液相色谱-四极杆飞行时间质谱(liquid chromatography-quadruple time-of-flight mass spectrometry,LCQTOF MS)结合正交偏最小二乘(orthogonal partial least square,OPLS)法分析不同贮存时间的冰鲜鸡肉(1、3、5、7、10 d)和鲜鸡肉(0 d)的代谢物,从中筛选出29个差异代谢物,主要包括蛋白质、脂肪和核苷酸降解产物。在冰鲜鸡肉贮存过程中,苯丙氨酸、色氨酸、棕榈酸和二十碳五烯酸含量显著上升,肌肽、鹅肌肽和肌苷酸等物质含量显著降低,并且发现贮存5 d以内的冰鲜鸡肉和鲜鸡肉在代谢物组成上具有更多的相似性。本实验表明,基于LC-QTOF MS技术的代谢组学方法结合OPLS模型对冰鲜鸡肉和鲜鸡肉的鉴别具有可行性,本研究为冰鲜鸡肉代谢物研究提供思路,为冰鲜鸡肉的质量控制及品质评价提供数据支撑。Liquid chromatography-quadruple time-of-flight mass spectrometry(LC-QTOF-MS)combined with orthogonal partial least square(OPLS)was used for metabolite analysis in chilled chicken(stored for 1,3,5,7 and 10 days)and fresh chicken.Twenty-nine differential compounds including amino acids,peptides,fatty acids and nucleotides were obtained in the experiment.With increasing storage period,the contents of phenylalanine,tryptophan,palmitic acid and eicosatetraenoic acid were significantly increased,while the contents of L-carnosine,L-anserine and 5’-IMP were significantly decreased.Moreover,chilled chicken stored for less than 5 days had more similarity with fresh chicken in metabolite composition.This study indicates that it is feasible to discriminate chilled from fresh chicken using metabolomics based on LC-QTOF-MS combined with OPLS model,which provides a novel idea for exploring metabolites in chilled chicken,and provides data support for the quality control and evaluation of chilled chicken.

关 键 词：冰鲜鸡肉 鲜鸡肉 贮存时间 代谢物 正交偏最小二乘法 

分 类 号：S831.9[农业科学—畜牧学]