甘薯渣酶解制备高纯度结晶葡萄糖和可溶性膳食纤维的研究  

The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

作　　者：石帅帅 席毅 倪贺 吕镇城 陈兆贵 李海航 SHI Shuaishuai;XI Yi;NI He;LÜZhencheng;CHEN Zhaogui;LI Haihang(School of Life Sciences∥Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China;School of Life Sciences, Huizhou University, Huizhou 516007, China)

机构地区：[1]华南师范大学生命科学学院/广东省植物发育生物工程重点实验室,广州510631 [2]惠州学院生命科学学院,惠州516007

出　　处：《华南师范大学学报（自然科学版）》2021年第4期61-67,共7页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省自然科学基金项目(2017A030310031)。

摘　　要：研究了甘薯渣酶解法制备葡萄糖及结晶生产高纯度结晶葡萄糖的工艺.新鲜甘薯渣加入1倍体积的水混匀,加入20 U/g的耐高温α-淀粉酶,90℃下液化60 min,冷却至室温后按300 U/g加入糖化酶,60℃下酶解6 h;酶解液加入1%的大孔树脂吸附除去杂质;糖溶液减压浓缩至葡萄糖质量分数为70%,65℃下加热30 min,加入1%的葡萄糖晶种,超声处理20 min后在4℃下结晶48 h;结晶出的葡萄糖用少量95%乙醇洗涤2次,真空烘干,所得葡萄糖的纯度为99%以上,结晶回收率达到90%.水解后剩余的甘薯渣加入150 U/g的纤维素酶,在pH 5.5、40℃下反应180 min.可溶性膳食纤维得率由2%提高到20%.该研究为甘薯渣的高效和高价值利用、减少资源浪费和环境污染提供简单而实用的方法.A practical method for preparing high-purity crystalline glucose through enzymolysis of sweet potato residue was developed.Freshly prepared sweet potato residue,mixed with water of equal volume,was first treated with 20 U/g thermostableα-amylase at 90℃for 60 min and then with 300 U/g glucoamylase at 60℃for 6 h.The hydrolyzed glucose solution was treated with 1%macroporous resin to remove the impurities,vacuum-concentrated to glucose content of 70%,and crystallized at 4℃for 48 h.After being washed twice with 95%ethanol and vacuum-dried,crystallized glucose with purity higher than 99%was obtained.After hydrolysis,the remaining sweet potato residue was mixed with 150 U/g cellulase and reacted at pH 5.5 and 40℃for 180 min.The yield of soluble dietary fiber increased from 2%to 20%.This research provides a simple and practical method for efficient utilization of sweet potato residue and reduction of resource waste and ecological pollution.

关 键 词：甘薯渣 酶解法 高纯度结晶葡萄糖 可溶性膳食纤维 

分 类 号：Q539[生物学—生物化学]