黑菊芋对小鼠肠道菌群及其短链脂肪酸产生的影响  

Effects of Black Jerusalem Artichoke on Intestinal Flora and Its Production of Short-chain Fatty Acids in Mice

作　　者：姬妍茹[1] 张正海[1] 杨庆丽[1] 滕春波[2] 董艳[1] 石杰[1] 刘玉[1] 魏连会[1] Ji Yanru;Zhang Zhenghai;Yang Qingli;Teng Chunbo;Dong Yan;Shi Jie;Liu Yu;Wei Lianhui(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang;College of Life Sciences,Northeast Forestry University,Haerbin 150040)

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319 [2]东北林业大学生命科学学院,哈尔滨150040

出　　处：《中国食品学报》2021年第8期159-165,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省院所基本应用技术研究专项(ZNBZ2019DQ03)。

摘　　要：为研究黑菊芋对小鼠肠道菌群及其短链脂肪酸产生的影响,将50只KM小鼠随机分为空白组、低聚果糖组、黑菊芋低、中、高剂量组,连续灌胃给药15 d,采用16S rDNA高通量测序和气相色谱-质谱技术分别检测小鼠肠道微生物组成和盲肠内容物中短链脂肪酸含量变化。结果显示:与空白组相比,黑菊芋不会影响小鼠的正常生长,能够调节小鼠肠道微生物组成,上调肠道免疫相关菌的相对丰度,并对拟杆菌科的未纯培养细菌f Muribaculaceae菌和毛螺菌科的NK4A136 group菌的生长有积极促进作用。同时,黑菊芋可以调节小鼠盲肠内容物中短链脂肪酸(SCFA)含量,增加丙酸、正丁酸和正戊酸的含量。这些结果表明,摄入适宜剂量(0.05~0.1 g)黑菊芋可以改善小鼠的肠道健康,增加抗炎能力。本研究为揭示黑菊芋的保健功能提供了理论依据。In order to study the effect of black Jerusalem artichoke on intestinal flora and its production of short-chain fatty acids in mice,50 KM mice were randomly divided into blank group,fructo-oligose group,black Jerusalem artichoke low,medium and high dose group.After continuous lavage for 15 days,changes of intestinal microbial composition and short-chain fatty acids content in cecum contents were detected based on 16S rDNA high-throughput sequencing and gas chromatography-mass spectrometry.The results showed that compared with the blank group,the black Jerusalem artichoke group did not affect the normal growth of mice,but could change the composition of intestinal bacteria of mice,regulate the relative abundance of some bacteria related to intestinal immunity,and promote the growth of uncultured bacterium f Muribaculaceae of Bacteroideaceae and NK4A136 group of Bacteroideaceae.Meanwhile,black Jerusalem artichoke could regulate the content of short-chain fatty acid(SCFA)in the cecum contents of mice and increase the content of propionic acid,n-butyric acid and n-valeric acid.It is suggested that taking appropriate dose(0.05-0.1 g)of black Jerusalem artichoke may improve the intestinal health of mice and increase the anti-inflammatory capacity.This study provides theoretical basis and basic data for the health function of black Jerusalem artichoke.

关 键 词：黑菊芋 肠道菌群 短链脂肪酸 KM小鼠 

分 类 号：TS201.4[轻工技术与工程—食品科学]