直接竞争酶联免疫吸附法检测食品中三聚氰胺残留  

Detection of melamine in food by direct competitive enzyme-linked immuno sorbent assay method

作　　者：樊晓博[1,2] FAN Xiao-Bo(Nursing College,Weinan Vocational&Technical College,Weinan 714000,China;Weinan Testing&Inspection and Research Center of Agricultural Products and Food,Weinan 714000,China)

机构地区：[1]渭南职业技术学院护理学院,渭南714000 [2]渭南市农产品食品检验检测研究中心,渭南714000

出　　处：《食品安全质量检测学报》2021年第14期5700-5706,共7页Journal of Food Safety and Quality

摘　　要：目的采用酶标抗原建立高效、高灵敏的三聚氰胺直接竞争酶联免疫吸附法(direct competitive enzyme linked immunosorbent assay,dc-ELISA)检测食品中三聚氰胺(melamine,MEL)残留。方法以三聚氰胺单克隆抗体包被作为固相抗体,辣根过氧化酶(horseradish peroxidase,HRP)标记的抗原与标准品(或样品)中三聚氰胺竞争结合抗体,建立了直接竞争酶联免疫检测体系,以三聚氰胺标准品建立标准曲线进行定量。结果方法的IC_(50)为8.84μg/L,灵敏度为0.65μg/L,线性范围0.9~35μg/L;检测样品的回收率在70%~120%之间,与三聚氰酸交叉反应率为60%,其他结构类似物基本无交叉反应。结论本方法灵敏度高、特异性强,可以满足实际样品的快速检测需求。Objective To establish a rapid,high sensitive method to detect melamine(MEL)residues in food by direct competitive enzyme linked immunosorbent assay(dc-ELISA).Methods The MEL antigen was labeled by horseradish peroxidase(HRP),monoclonal antibody was bounded on the surface of a micro titer plate,the standards(or the samples)competed with MEL antigen for the antibody binding sites,a dc-ELISA was established,and the standard curve of melamine was prepared for quantitative analysis.Results The IC_(50) of dc-ELISA was 8.84μg/L,the limit of detection was 0.65μg/L,the linear detection ranges were 0.9‒35μg/L,the recoveries of all kinds of samples were range from 70.0%to 120%,and the cross reaction with cyanuric acid was 60%,and there was almost no cross reaction with other drugs with similar construction.Conclusion The established method is sensitive and specific,which can meet the rapid detection demand of actual samples.

关 键 词：三聚氰胺 酶标抗原 直接竞争酶联免疫吸附法 

分 类 号：TS207.3[轻工技术与工程—食品科学]