产酸性纤维素酶细菌的筛选鉴定及其酶学性质  

Screening, Isolating and Enzymatic Properties of Bacterium Producing Acidic Cellulase

作　　者：孙会刚[1] 徐慧敏 黄天姿[1] 陈学红[1] 李勇[1] 李同祥[1] SUN Huigang;XU Huimin;HUANG Tianzi;CHEN Xuehong;LI Yong;LI Tongxiang(College of Food and Biological Engineering,Xuzhou University of Technology,Xuzhou 221018,China)

机构地区：[1]徐州工程学院食品与生物工程学院,江苏徐州221018

出　　处：《食品科技》2021年第7期55-59,共5页Food Science and Technology

基　　金：江苏省高校自然科学基金重大项目(19KJA480002);徐州市科技计划项目(KC20186);徐州市重点研发计划项目(KC20045)。

摘　　要：采用刚果红染色法进行初筛,3,5-二硝基水杨酸法进行复筛,从不同来源的土壤样品中筛选到了一株产纤维素酶活性较高的菌株,酶活达到39.20 U/mL,命名为XH-4。通过形态学观察、生理生化及分子生物学试验,初步确定该菌株为贝莱斯芽孢杆菌。对其产生的纤维素酶酶学性质研究表明:纤维素酶的最适反应温度为50℃,最适反应pH值为6,在30~55℃、pH值为3~6时具有较好的稳定性。金属离子Cu^(2+)、Ag^(+)、Mn^(2+)、Zn^(2+)、Al3+、Mn^(2+)对该纤维素酶活性有促进作用,其中Ag+对纤维素酶活性促进作用最强;Ca^(2+)、Fe^(3+)、K+对其活性有抑制作用。In this study,the strain producing high activity cellulase was isolated from some samples by Congo red staining method and 3,5-dinitrosalicylic acid method.The strain producing highest activity cellulase,named XH-4 was further analyzed.XH-4 was identified as Bacillus velezensis.The optimum temperature of cellulase from XH-4 is 50 ℃,and the enzyme activity reached at 39.20 U/mL.The optimum pH is 6.0.The enzymatic properties analysis showed that enzyme activity is stable at 30~55 ℃ and pH3~6.The enzyme activity was promoted by Cu^(2+),Ag^(+),Mn^(2+),Zn^(2+),Al^(3+),Mn^(2+) and inhibited by Ca^(2+)、Fe^(3+) and K^(+).Among them,Ag+ have the strongest promoting effect.

关 键 词：纤维素酶 分离和鉴定 贝莱斯芽孢杆菌 酶学性质 

分 类 号：TS201.3[轻工技术与工程—食品科学]