不同蛋白种类的膳食对结肠炎小鼠的影响  

Effects of different protein diets on mice with colitis

作　　者：方淼 吴秀文[2] 郑涛 龚文斌 任建安 Fang Miao;Wu Xiuwen;Zheng Tao;Gong Wenbin;Ren Jianan(Jinling Clinical Medical College,Nanjing Medical University,Nanjing 210009,China;Department of General Surgery,Jinling Hospital,Nanjing University,School of Medicine,Nanjing 210002,China)

机构地区：[1]南京医科大学金陵临床医学院,南京210009 [2]解放军东部战区总医院(原南京军区南京总医院)普通外科,南京210002

出　　处：《中华炎性肠病杂志（中英文）》2021年第3期225-228,共4页Chinese Journal of Inflammatory Bowel Diseases

基　　金：江苏省333高层次人才培养工程(BRA2019011);后勤科研项目(CLB19J025)。

摘　　要：目的比较酪蛋白、乳清蛋白和大豆蛋白型膳食对结肠炎小鼠的影响。方法50只C57BL/6小鼠随机分为5组,分别为空白对照组、一般饮食组、乳清蛋白组、大豆蛋白组及酪蛋白组,每组10只。以4种不同蛋白种类的纯化膳食连续喂养小鼠14 d,除空白对照组以外,其余4组在实验的第7天以葡聚糖硫酸钠(DSS)诱导急性结肠炎。期间观察并记录各组小鼠的疾病活动度评分。第14天取各组小鼠血清及结肠组织,以ELISA法检测血清和结肠组织的IL-6、TNF-α和IL-10水平,并计算结肠病理评分。结果乳清蛋白组疾病活动度评分明显低于酪蛋白组和大豆蛋白组(P<0.05),高于空白对照组(P<0.05)。空白对照组的结肠病理评分平均低于其余4组(P均<0.05)。乳清蛋白组结肠组织病理评分低于酪蛋白和大豆蛋白组(P<0.05),与一般饮食组无显著差异。乳清蛋白组血清IL-6和TNF-α水平明显低于酪蛋白和大豆蛋白组(P<0.05),IL-10水平明显高于一般饮食、酪蛋白及大豆蛋白组(P<0.05),结肠IL-10水平明显高于酪蛋白组和大豆蛋白组(P<0.05)。结论乳清蛋白型膳食用以缓解小鼠急性结肠炎的治疗效果明显优于一般饮食、酪蛋白及大豆蛋白型膳食。Objective To compare the effects of casein,whey and soy protein diet on treating colitis.Methods Fifty C57BL/6 mice were randomly and equally divided into control group,general diet group,casein group,whey group and soy group with 10 mice in each group.Mice were fed with corresponding diets for 14 days.Acute colitis mice modelof general diet group,casein group,whey group and soy group were induced by dextran sulfate sodium(DSS)on the 7th day.The disease activity index(DAI)score was evaluated and recorded.The serum and colon tissues of each group on the 14th day were collected todetect the serum levels of IL-6,TNF-αand IL-10 by ELISA,and to calculate the pathological score of colon.Results The DAI score of the whey group was significantly lower than that of the casein group and soy protein group(all P<0.05),and higher than that of the control group(P<0.05).The pathological score of colon tissue of the control group was significantly lower as compared to other 4 groups(all P<0.05),while above scoreof whey group was significantly lower than that of the casein group and soy protein group(all P<0.05),but there was no significant difference between the whey group and general diet group.The levels of serum IL-6 and TNF-αin were significantly lower than those in the casein group and soy group(all P<0.05),and serum IL-10 level in the whey group was significantly higher than that in general diet,casein and soy groups(all P<0.05).The level of colon IL-10 in the whey group was significantly higher than that in the casein group and soy group(all P<0.05).Conclusion The efficacy of whey protein diet on treating acute colitis mice is better compared to general diet,casein and soy protein diet.

关 键 词：蛋白 膳食 结肠炎 肠内营养 

分 类 号：R574.62[医药卫生—消化系统]