适应性驯化生产低分子质量β-葡聚糖及其抗氧化活性研究  

Improvement of low-massβ-glucan yield by adaptive laboratory evolution and its antioxidant activity

作　　者：王冰 朱莉 李茂玮 詹晓北[1] WANG Bing;ZHU Li;LI Maowei;ZHAN Xiaobei(Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China;Wuxi Galaxy Biotech Co.Ltd.,Wuxi 214125,China)

机构地区：[1]糖化学与生物技术教育部重点实验室,江南大学生物工程学院,江苏无锡214122 [2]无锡格莱克斯生物科技有限公司,江苏无锡214125

出　　处：《食品与发酵工业》2021年第17期27-33,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31171640)。

摘　　要：β-葡聚糖被认为是一种潜在的功能性多糖。利用适应性驯化(adaptive laboratory evolution,ALE)的方法,将甘油作为碳源,诱导土壤杆菌生产低分子质量β-葡聚糖,研究新型多糖的抗氧化活性。通过筛选获得了1株将甘油作为碳源、可稳定获得低分子质量产物的高活力菌株Agrobacterium sp.WS-8E3T,该菌株在100 g/L甘油中摇瓶发酵120 h,稳定产生分子质量在2800~3600 Da的新型低分子质量多糖(β-gluco-oligosaccharides,BGOs)。通过红外光谱、单糖组成及核磁分析对其结构进行解析,证明BGOs为β-1,3-葡聚糖。该葡聚糖显示了一定的抗氧化活性,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)清除率、·OH清除率均能达到抗坏血酸的80%。采用甘油适应性驯化菌株的方法生产得到的低分子质量β-葡聚糖具有较好的抗氧化性能,研究结果为BGOs在功能食品和医药领域的应用提供了研究基础。β-Glucan is considered as a potential functional polysaccharide.The adaptive laboratory evolution was used to induce Agrobacterium sp.to produce low molecular weightβ-glucan with glycerol,and the antioxidant activity was determined.Agrobacterium sp.WS-8E3T produced,a novel polysaccharide,β-gluco-oligosaccharides(BGOs)with 2800-3600 Da stably after 120 h in 100 g/L glycerol shake flask.The BGOs was analyzed by infrared spectrum,monosaccharide composition and NMR,which proved that BGOs wasβ-1,3-glucan.The BGOs had antioxidant capacity with the scavenging rate of DPPH radical and hydroxyl radical reached to 80%of ascorbic acid.The results showed that the BGOs had better antioxidant properties,and provides the basis for the application of BGOs in the functional food and medicine field.

关 键 词：Β-葡聚糖 甘油 适应性驯化 结构表征 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]