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作 者:李洪媛 马美荣[1] 周林艳 刘天成 刘小改[1] 王小伟[1] 张坤[1] LI Hongyuan;MA Meirong;ZHOU Linyan;LIU Tiancheng;LIU Xiaogai;WANG Xiaowei;ZHANG Kun(Beijing Red Star Co.Ltd.,Beijing 101400,China)
出 处:《酿酒科技》2021年第9期123-130,共8页Liquor-Making Science & Technology
摘 要:选用从清香型白酒大曲、酒醅中分离得到的酵母、根霉菌种进行性能研究实验,筛选得到生长速率较为适宜且产乙酸乙酯能力突出的酵母菌,高产糖化酶和酯化酶的根霉菌。通过酿酒小试实验确定根霉酵母菌种的组合以及制曲采用混合培养方式。经过中试实验,确定了适用于圆盘制曲培养的参数条件,成曲通过多排次生产发酵实验进行验证。结果显示,圆盘混合麸曲中酵母数量可达32×108个/g麸曲,糖化力在500 U左右,其应用于麸曲清香型白酒生产,原酒乙酸乙酯含量由0.90 g/L提升到1.52 g/L。In this paper,Rhizopus and Saccharomyces strains isolated from Daqu and fermented grains of Qingxiang Baijiu were selected for performance tests.Saccharomyces strains with suitable growth rate and outstanding ethyl acetate production capacity,and Rhizopus strains with high yield of glucoamylase and esterase were screened out.The combination of Rhizopus and Saccharomyces strains and the mixed culture method were determined through small-scale fermentation experiments.Then pilot experiments were carried out to determine the Fuqu-making parameters by disc culture.The performance of the produced Fuqu was verified by a series of production tests.The results showed that the number of Saccharomyces in the produced Fuqu reached 3.2 billion/g Fuqu,and the sac-charification power was about 500 U;when used in the production of Qingxiang Baijiu,the ethyl acetate content in the base liquor in-creased from 0.90 g/L to 1.52 g/L.
分 类 号:TS262.3[轻工技术与工程—发酵工程] TS261.1[轻工技术与工程—食品科学与工程]
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