酶解豆渣工艺及其在黄原胶发酵中的应用  

Enzymatic hydrolysis of soybean dregs and its application in xanthan gum fermentation

作　　者：李学朋 陈晓妮 刘超 张培鑫 徐娜 周敬 LI Xuepeng;CHEN Xiaoni;LIU Chao;ZHANG Peixin;XU Na;ZHOU Jing(Inner Mongolia Fufeng Biotechnology Co.,Ltd.,Hohhot 010030,China;Inner Mongolia Key Laboratory of Bio FermentationEnergy Saving and Environmental Protection Technology Enterprises,Hohhot 010030,China)

机构地区：[1]内蒙古阜丰生物科技有限公司,内蒙古呼和浩特010030 [2]内蒙古自治区生物发酵节能环保技术企业重点实验室,内蒙古呼和浩特010030

出　　处：《发酵科技通讯》2021年第3期132-136,共5页Bulletin of Fermentation Science and Technology

摘　　要：黄原胶生产过程中的副产物豆渣仍含有大量未被利用的营养物质,造成了资源的浪费。利用单因素实验和正交实验确定蛋白回收的最佳条件:酶解温度50℃,酶解时间5 h,最佳复合酶为碱性蛋白酶和纤维素酶复配,最佳酶使用量为4%。该条件下,大豆蛋白的回收率可达90%以上。将回收的大豆蛋白用于黄原胶发酵,对黄原胶的产胶及转化率无影响,但可提高黄原胶的黏度。As a by-product of xanthan gum fermentation,bean dregs still contain a large amount of unused nutrients,resulting in a waste of resources.Single factor test and orthogonal test were used to determine the best conditions for protein recovery,which are 50℃enzymolysis temperatureand 5 h enzymatic hydrolysis time.The best complex enzyme is the complex of alkaline protease and cellulose,and the optimum enzyme dosage is 4%.Under these conditions,the recovery rate of soybean protein can reach more than 90%.When the recovered soybean protein was used in xanthan gum fermentation,the yield of xanthan gum and the conversion of soybean were not affected,but the viscosity of xanthan gum was improved.

关 键 词：豆渣 蛋白回收率 酶解 黄原胶 

分 类 号：Q819[生物学—生物工程]