苯丙氨酸-肌酐模型反应体系中甘蔗糖蜜酚酸单体对PhIP的抑制作用  

Inhibitory Effects of Phenolic Acid Monomers in Sugarcane Molasses on PhIP in a Phenylalanine-creatinine Model Reaction System

作　　者：于迪 龙娟 黄媛 黄嘉佳 赵立春 孔繁磊 YU Di;LONG Juan;HUANG Yuan;HUANG Jia-jia;ZHAO Li-chun;KONG Fan-lei(College of Pharmacy,Guangxi University of Chinese medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200

出　　处：《现代食品科技》2021年第9期86-92,192,共8页Modern Food Science and Technology

基　　金：国家自然科学基金项目(32060576);广西科技计划项目(AD18281081,2018GXNSFAA294078);广西教育厅中青年教师基础能力提升项目(2018KY0272);广西中医药大学博士科研启动项目(2017BS030);广西中医药大学校级课题(2018MS004);广西中医药大学“岐黄工程”高层次人才团队培育项目(2018002);广西高等学校高水平创新团队及卓越学者项目(2019-52)。

摘　　要：该研究探讨了模型反应体系中甘蔗糖蜜含有的阿魏酸和香草酸对2-氨基-1-甲基-6-苯基-咪唑[4,5-b]吡啶(PhIP)的抑制作用。实验通过糖蜜净化处理方法去除糖蜜中的胶体、灰分和重金属等物质,得到糖蜜粗提物,建立没食子酸标准曲线,测定糖蜜提取物中多酚含量。通过UPLC-MS对糖蜜提取物进行分析,确定糖蜜提取物中含有的酚酸种类。将酚酸单体分别加入到模型反应(苯丙氨酸和肌酐)体系中,用UPLC-MS系统分析模型反应中PhIP的变化。结果发现,糖蜜提取物中多酚含量多达3.58 mg/g,通过和酚酸标准品对照,发现糖蜜提取物中含有阿魏酸和香草酸。在模型反应体系中,随着阿魏酸和香草酸浓度的增加,模型反应中苯丙氨酸含量和PhIP的生成量均先逐渐减少后趋于平稳,当阿魏酸溶液浓度达到2.33×10^(-7) g/mL时,对PhIP的抑制效果最佳,总体抑制率为76.67%;当香草酸溶液浓度达到2.02×10^(-7) g/mL时,对PhIP的抑制效果最佳,总体抑制率为77.43%。综上,甘蔗糖蜜中含有的阿魏酸和香草酸单体在模型反应体系中均对PhIP有较强的抑制作用,为后续对PhIP抑制物的研究提供了基础。The inhibitory effect of ferulic acid and vanillic acid in sugarcane molasses on PhIP was studied in a model reaction system.The colloid,ash and heavy metals in the molasses were removed through the molasses purification treatment method to yield a crude molasses extract.A gallic acid standard curve was established to determine the polyphenol content in molasses extract.The type of phenolic acids in the molasses extract was determined by UPLC-MS analysis.The phenolic acid standard was added to the model reaction system(phenylalanine and creatinine),and changes in PhIP in the model reaction system was analyzed by the UPLC-MS/MS system.The results showed that the polyphenol content in the molasses extract was as high as 3.58 mg/g.Compared with the phenolic acid standard,the molasses extract was found to contain ferulic acid and vanillic acid.In the model reaction system,with the increase of the concentration of vanillic acid or ferulic acid,both the phenylalanine content and the amount of PhIP produced in the model reaction first decreased gradually and then leveled off.When the concentration of ferulic acid reached 2.33×10^(-7) g/mL,the inhibitory effect on PhIP was the best,and the overall inhibitory rate was 76.67%.When the concentration of vanillic acid reached 2.02×10^(-7) g/mL,the inhibitory effect on PhIP was the greatest,and the overall inhibitory rate reached 77.43%.In summary,the ferulic acid and vanillic acid monomers contained in sugarcane molasses had strong inhibitory effects on PhIP in the model reaction system,which provides a basis for subsequent research on the inhibitors of PhIP.

关 键 词：杂环胺 2-氨基-1-甲基-6-苯基-咪唑[4 5-b]吡啶(PhIP) 甘蔗糖蜜 酚酸 

分 类 号：TS201.2[轻工技术与工程—食品科学]