水分活度对轻腌大黄鱼源蜂房哈夫尼菌碳源利用能力的影响  

Effects of water activity on carbon source utilization ability of Hafnia alvei isolated from lightly salted Larimichthys crocea

作　　者：王晓阳 郭全友[2] 黄海潮 杨絮 张小敏 WANG Xiaoyang;GUO Quanyou;HUANG Haichao;YANG Xu;ZHANG Xiaomin(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090 [3]上海理工大学医疗器械与食品学院,上海200093

出　　处：《食品与发酵工业》2021年第18期37-45,共9页Food and Fermentation Industries

基　　金：国家自然科学基金(31871872);中国水产科学研究院基本科研业务费资助项目(2020TD68);中央级公益性科研院所基本科研业务费专项资金(2018M04)。

摘　　要：为探究水分活度(water activity,A w)对轻腌大黄鱼源蜂房哈弗尼菌碳源代谢的影响,利用Biolog GENⅢ微孔板测定25℃、不同A w(0.92、0.93、0.94、0.95、0.96、0.97、0.98)下该菌碳源利用情况,采用修正的Gompertz模型拟合代谢曲线,获取动力学参数,结合平均颜色变化率及利用面积,探究其不同A w下碳源利用效果及动力学特征。结果表明,不同A w(0.92~0.98)下蜂房哈弗尼菌羧酸类(37.3%)和氨基酸类(32.6%)利用率均值较高,A w为0.98时菌株总体碳源利用能力最强,0.96时次之,剩余组由强到弱依次为0.95、0.92、0.93、0.94、0.97。各A w下菌株生长利用的优势单一碳源主要有L-岩藻糖、D-岩藻糖、L-鼠李糖、D-葡糖醛酸、乙酰乙酸、乙酸、D-丝氨酸、L-丙氨酸、L-天冬氨酸、N-乙酰-D-葡糖胺、L-组氨酸、D-葡糖-6-磷酸和D-果糖-6-磷酸。随着A w升高,菌株碳源利用最大比生长速率(μmax)和迟滞期(λ)分别呈现增大和减小的趋势。通过调控底物碳源及环境因子等手段,探究蜂房哈弗尼菌碳源利用和生长动力学,可为靶向抑菌及大黄鱼产品工艺优化提供一定支撑。To explore the effect of water activity(A w)on the carbon metabolism of Hafnia alvei from lightly salted Larimichthys crocea,the carbon source utilization of the strain was measured by Biolog GENⅢmicroplate under different A w(0.92,0.93,0.94,0.95,0.96,0.97,0.98)at 25℃.The modified Gompertz model was used to fit the metabolic curve and obtain the kinetic parameters.And combining the average well color development(AWCD)and the utilization area,the utilization effect and dynamic characteristics of carbon source under different A w were explored.The results showed that for H.alvei,the average utilization rate of carboxylic acids(37.3%)and amino acids(32.6%)was higher under different A w(0.92-0.98).The overall carbon source utilization capacity was the strongest at A w of 0.98,followed by 0.96.And the remaining group from strong to weak was 0.95,0.92,0.93,0.94 and 0.97.The dominant single carbon sources for the growth of H.alvei were mainly L-fucose,D-fucose,L-rhamnose,D-glucuronic acid,acetoacetic acid,acetic acid,D-serine,L-alanine,L-aspartic acid,N-acetyl-D-glucosamine,L-histidine,D-glucose-6-PO 4 and D-fructose-6-PO 4 at A w of 0.92-0.98.With the increase of A w,the maximum specific growth rate(μmax)and lag phase duration(λ)of the carbon source utilization of H.alvei showed the trend of increasing and decreasing respectively.Through regulating the carbon source of substrate and environmental factors,the carbon source utilization and growth dynamics of H.alvei from the lightly salted L.crocea were explored,which could provide certain support for targeting bacteriostasis and process optimization of L.crocea.

关 键 词：轻腌大黄鱼 特定腐败菌 蜂房哈弗尼菌 碳源利用 水分活度 生长动力学 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]