高温淀粉酶高产菌株的ARTP诱变选育及发酵条件优化  

Screening of High-Temperature Amylase High-Yielding Strains by ARTP Mutation and Optimization of Fermentation Conditions

作　　者：张晋 宇海萍[2] 马磊磊 冯志强 ZHANG Jin;YU Haiping;MA Leilei;FENG Zhiqiang(Jinzhong College of Information,Taigu 030800,China;College of Information Science and Engineering,Shanxi Agricultural University,Taigu 030801,China;Shandong Longkete Enzyme Co.,Ltd.,Linyi 276400,China;College of Food and Biological Engineering(Liquor College),Henan University of Animal Husbandry and Economy,Zhengzhou 450000,China)

机构地区：[1]晋中信息学院,山西太谷030800 [2]山西农业大学信息科学与工程学院,山西太谷030801 [3]山东隆科特酶制剂有限公司,山东临沂276400 [4]河南牧业经济学院食品与生物工程学院(酒业学院),河南郑州450000

出　　处：《食品科技》2021年第8期22-26,共5页Food Science and Technology

基　　金：河南省重大科技专项(181100211400)。

摘　　要：利用常压室温等离子体(ARTP)诱变仪对产高温淀粉酶重组毕赤酵母进行诱变处理,并通过台盼蓝平板显色法初筛出产酶优势菌株,再通过摇瓶复筛选育出一株高温淀粉酶高产菌株A28,其酶活力达到1165 U/mL,较初始菌株酶活力提高37%。并对该菌株的发酵周期、接种量、诱导温度及甲醇添加量进行优化,得到最佳发酵时长为7 d、接种量为8%、诱导温度为26 ℃、甲醇添加量为1%/d,在该条件下,发酵酶活达到1835 U/mL。在连续10代的传代培养中,其酶活力保持在1830 U/mL左右,具有重要的工业应用价值。The high-temperature amylase-producing recombinant Pichia pastoris was mutagenized with an atmospheric pressure room temperature plasma (ARTP) mutagenizer,and the dominant enzyme-producing strains were initially screened by the Trypan blue plate color method,and then re-screened by shaking flasks.A high-temperature amylase high-yielding strain A28 was bred,and its enzyme activity reached 1165 U/mL,which was 37% higher than the initial strain.And fermentation period of the strain,induced temperature,inoculation quantity,and the amount of methanol added were optimized.The best fermentation time was 7 days,the inoculum was 8%,the induction temperature was 26 ℃,and the methanol addition was 1%/d.Under these conditions,the fermentation enzyme activity reached 1835 U/mL.In the subculture for 10 consecutive generations,its enzyme activity remained at about 1830 U/mL,It has important industrial application value.

关 键 词：常压室温等离子体 高温淀粉酶 发酵优化 毕赤酵母 

分 类 号：TS201.3[轻工技术与工程—食品科学]