Baroreflex deficiency aggravates learning and memory disorders in rats  

作　　者：ZHU De-rong FENG Zhao-yang JI Wei QI Hui-bin KONG De-ping ZHANG Fang-fang YU Hai-yang GAO Yong-feng TAN Rui ZHAO Xiao-min 

机构地区：[1]Institute of Pharmacology,Shandong First Medical University&Shandong Academy of Medical Sciences,Tai′an 271016,China

出　　处：《中国药理学与毒理学杂志》2021年第9期649-650,共2页Chinese Journal of Pharmacology and Toxicology

基　　金：Natural Science Foundation of Shandong Province(ZR2017MH048)。

摘　　要：OBJECTIVE Microglia M1/M2 po⁃larization play pro-inflammatory and anti-inflam⁃matory roles,respectively,which is involved in memory decline.There is a close relationship between impaired baroreflex function and memory impairment.The present study was designed to investigate whether arterial baroreflex deficiency induced by sinoaortic denervation(SAD)affected inflammation through modulation of M1/M2 polar⁃ization leading to the aggravation of learning and memory disorders in rats.METHODS Adult male SD rats were divided into four groups:the sham control,the SAD,the sham+scopolamine,the SAD+scopolamine.In another experiment,there were also four groups:the sham control,the SAD,the SAD+scopolamine and the SAD+scopolamine+ketanserin.All rats were examined for various behaviors using Morris water maze test,new object recognition test,and light dark shuttle test and Y maze test 4 weeks after sham or SAD surgery.CD16,CD206,IL-10,IL-6,IL-1βand TNF-αfrom hippocampus using Western blotting,immunofluorescence and turbidimetry.RESULTS Compared with the sham+scopol⁃amine,the SAD+scopolamine rats showed the reduced crossing times in Morris water maze test,the longer residence time in dark box during light dark shuttle test,and the decreased alterna⁃tion ratio in Y maze test.The level of CD206,IL-10,T-AOC and GSH was decreased,whereas CD16,IL-6,TNF-α,MDA was increased in the hippocampus of SAD+scopolamine rats.Addi⁃tionally,all the above changes were improved in the SAD+scopolamine+ketanserin rats when compared with the SAD+scopolamine.CONCLU⁃SION Arterial baroreflex dysfunction aggravates learning and memory disorders in rats,which may be related to the polarization of microglia.

关 键 词：arterial baroreflex learning and memory MICROGLIA POLARIZATION KETANSERIN 

分 类 号：R741.02[医药卫生—神经病学与精神病学]