大粒车前子多糖结构特征对调节小鼠肠道功能的影响  

Effect of Structural Characteristics of Polysaccharides from Seeds of Plantago asiatica L.on the Regulation of Intestinal Function in Mice

作　　者：殷军艺[1] 刘晓莹[1,2] 马璐瑶 王力[1] 邹明悦[1] 聂少平 Yin Junyi;Liu Xiaoying;Ma Luyao;Wang Li;Zou Mingyue;Nie Shaoping(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047;Shaanxi Xifeng Liquor Company Limited,Baoji 721400,Shaanxi)

机构地区：[1]食品科学与技术国家重点实验室南昌大学,南昌330047 [2]陕西西凤酒股份有限公司,陕西宝鸡721400

出　　处：《中国食品学报》2021年第9期79-88,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金杰出青年基金项目(31825020);江西省杰出青年人才资助计划项目(20192BCB23005)。

摘　　要：车前子多糖具有较好的肠道调节功能,然而,有关不同结构特征对调节肠道功能影响研究很少。本文以大粒车前子为研究对象,从中分别提取高黏性阿拉伯木聚糖(PLCP),低黏性阿拉伯木聚糖(PLCP-M)和(PLWE-H),以及非阿拉伯木聚糖类多糖组分PLWE-L,利用体内动物试验结合相关肠道健康功能指标分析,明确大粒车前子不同结构类型多糖对其肠道功能的影响。结果表明,非阿拉伯木聚糖(PLWE-L)的摄入可显著增加小鼠结肠、盲肠内容物中短链脂肪酸的浓度,同时增加粪便含水量。高黏度的PLCP更容易在结肠中发挥作用,包括促进结肠中总短链脂肪酸浓度的增加及降低pH值,同时有利于提高持水力,而低黏性组分PLCP-M和PLWE-L更易在盲肠中发挥相应的作用。车前子中不同结构特征组分在调节小鼠肠道功能方面存在差异。本研究将为车前子多糖肠道健康功能的深入研究提供理论支持,以期为进一步开发相关肠道保健产品提供理论依据。Polysaccharides from Plantago asiatica L have good activities in intestinal function regulation,but there are few reports on their different effects because of the diverse structure.In this paper,high viscous arabinoxylan(PLCP),low viscous arabinoxylan(PLCP-M and PLWE-H),and non-arabinoxylan(PLWE-L)were prepared from the seeds of Plantago asiatica L.Animal experiments were applied to elucidate their effects on the intestinal function regulation of mice.The results showed that the intake of PLWE-L significantly increased the concentration of SCFA in colon and cecum contents,and increased the water content of feces.High viscous PLCP was more likely to play a role in the colon,which included increasing the concentration of total SCFAs in the colon,lowering pH value and improving water retention.While the low viscous components(PLCP-M and PLWE-L)probably played certain effects in the cecum.Therefore,there are some differences in effects of the four fractions on intestinal function regulation in mice.This study would provide theoretical support for the in-depth study on intestinal health of Plantago asiatica L.polysaccharide,and provide theoretical basis for the further development of related products.

关 键 词：大粒车前子 多糖 结构 肠道功能 短链脂肪酸 

分 类 号：TS201.4[轻工技术与工程—食品科学]