基于高通量测序技术分析不同地区自然陈化广陈皮表面微生物群落多样性  

Microbial community diversity on the surface of naturally aged Pericarpium Citri Reticulatae'Chachi'in different regions based on high-throughput sequencing technology

作　　者：杨放晴 何丽英 杨丹 申梦园 王福[1] 陈鸿平[1] 刘友平[1] YANG Fangqing;HE Liying;YANG Dan;SHEN Mengyuan;WANG Fu;CHEN Hongping;LIU Youping(College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《中国酿造》2021年第9期31-36,共6页China Brewing

基　　金：国家自然科学基金项目(81973436)。

摘　　要：采用高通量测序技术对不同地区自然陈化1年广陈皮表面微生物群落多样性进行分析,比较群落结构的差异。结果表明,不同地区自然陈化1年广陈皮微生物丰富度和多样性差异不显著(P>0.05),群落结构相似,但相对丰度存在差异。北京地区优势细菌属为Methylocella、苔藓杆菌属(Bryobacter)等,优势真菌属为短梗霉属(Aureobasidium)、炭疽菌属(Colletotrichum)等;四川地区优势细菌属为甲基杆菌属(Methylobacterium)、鞘氨醇单胞菌属(Sphingomonas)、芽孢杆菌属(Bacillus)、乳球菌属(Lactococcus)等,优势真菌属为Symmetrospora、曲霉属(Aspergillus)等;广东地区优势细菌属为Rubellimicrobium、乳杆菌属(Lactobacillus)等,优势真菌属为假尾孢属(Pseudocercospora)、毛壳菌属(Chaetomium)等;云南地区优势细菌属为罗尔斯顿菌属(Ralstonia)、Glutamicibacter等,优势真菌属为平脐疣孢属(Zasmidium)、拟暗球腔菌属(Phaeosphaeriopsis)等。Microbial community diversity on the surface of Pericarpium Citri Reticulatae'Chachi'(tangerine peel)naturally aged 1 year from different regions was analyzed based on high-throughput sequencing technology,and the difference of community structure was compared.The results showed that there were no significant differences in the microbial richness and diversity of'Chachi'naturally aged 1 year from different regions(P>0.05),and the community structure was similar,but the relative abundance was different.The dominant bacteria genera in Beijing area were Methylocella,Bryobacter and so on,and the dominant fungi genera were Aureobasidium,Colletotrichum and so on.The dominant bacteria genera in Sichuan area were Methylobacterium,Sphingomonas,Bacillus,Lactococcus and so on,and the dominant fungi genera were Symmetrospora,Aspergillus and so on.The dominant bacteria genera in Guangdong area were Rubellimicrobium,Lactobacillus and so on,and the dominant fungi genera were Pseudocercospora,Chaetomium and so on.The dominant bacteria genera in Yunnan area were Ralstonia,Glutamicibacter and so on,and the dominant fungi genera were Zasmidium,Phaeosphaeriopsis and so on.

关 键 词：广陈皮 自然陈化 细菌多样性 真菌多样性 高通量测序 

分 类 号：TS201.3[轻工技术与工程—食品科学]