赊店老酒大曲中可培养真菌的分离和鉴定  

Isolation and identification of culturable fungi in Shedianlaojiu Daqu

作　　者：刘延波[1,2,3,4] 张达 卢倩倩 韩素娜 王贤 孙西玉 潘春梅[1,3,4] LIU Yanbo;ZHANG Da;LU Qianqian;HAN Su'na;WANG Xian;SUN Xiyu;PAN Chunmei(College of Food and Biological Engineering(Liquor College),Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Postdoctoral Programme,Henan Yangshao Distillery Co.,Ltd.,Mianchi 472400,China;Henan Liquor Style Engineering Technology Research Center,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Zhengzhou Key Laboratory of Liquor Brewing Microbial Technology,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;SheDianLaoJiu Co.,Ltd.,Sheqi 473300,China;Henan ZhangGongLaoJiu Wine Co.,Ltd.,Ningling 476733,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院(酒业学院),河南郑州450046 [2]河南仰韶酒业有限公司博士后科研工作站,河南渑池472400 [3]河南牧业经济学院河南省白酒风格工程技术研究中心,河南郑州450046 [4]河南牧业经济学院郑州市白酒酿造微生物技术重点实验室,河南郑州450046 [5]赊店老酒股份有限公司,河南社旗473300 [6]河南张弓老酒酒业有限公司,河南宁陵476733

出　　处：《中国酿造》2021年第9期76-81,共6页China Brewing

基　　金：河南省重大科技专项(181100211400);河南省重点研发与推广专项(科技攻关)项目(202102110130);河南牧业经济学院博士科研启动资金项目(2018HNUAHEDF011);河南牧业经济学院重点学科项目。

摘　　要：该研究采用传统分离培养方法,对赊店老酒大曲中的真菌进行分离纯化并通过形态学观察及分子生物学鉴定确定所获菌株的分类地位。结果表明,老酒大曲样品中共计分离得到45株酵母菌和103株霉菌。共鉴定出6株酵母菌,分别为酿酒酵母(Saccharomyces cerevisiae)、异常威克汉姆酵母(Wickerhamomyces anomalus)、扣囊复膜酵母(Saccharomycopsis fibuligera)、发酵毕赤酵母(Pichia fermentans)、胶红酵母(Rhodotorula mucilaginosa)及光滑假丝酵母(Candida glabrata);酵母的优势菌属为酿酒酵母(Saccharomyces cerevisiae)(相对含量71%)、光滑假丝酵母属(Candida glabrata)(相对含量11%)。共鉴定出4个霉菌属,分别为根霉属(Rhizopus)、横梗霉属(Lichtheimia)、曲霉属(Aspergillus)和毛霉属(Mucor);霉菌的优势菌属为根霉属(Rhizopus)(相对含量37%)、横梗霉属(Lichtheimia)(相对含量33%)。Fungi were isolated and purified from Shedianlaojiu Daqu by traditional isolation and culture methods,and the classification status of the obtained strains was determined through morphological observation and molecular biology identification.The results showed that a total of 45 yeasts and 103 molds were isolated from the Shedianlaojiu Daqu samples.A total of 6 yeasts were identified,namely Saccharomyces cerevisiae,Wickerhamomyces anomalus,Saccharomycopsis fibuligera,Pichia fermentans,Rhodotorula mucilaginosa and Candida glabrata,and the dominant genera of yeast were S.cerevisiae(relative content 71%)and C.glabrata(relative content 11%).A total of 4 molds were identified,namely Rhizopus,Lichtheimia,Aspergillus and Mucor,and the dominant genera of molds were Rhizopus(relative content 37%)and Lichtheimia(relative content 33%).

关 键 词：赊店老酒 大曲 真菌 分离鉴定 物种多样性 

分 类 号：TS261.1[轻工技术与工程—发酵工程]