响应面法优化托盘根总皂苷提取工艺及抗氧化活性研究  

Optimization of Extraction Process of Total Saponins from Rubus crataegifolius Bunge.Root by Response Surface Methodology and Its Antioxidant Activity

作　　者：杨秀东[1] 白子凡 徐永涛 梁倍 周鸿立[1] YANG Xiudong;BAI Zifan;XU Yongtao;LIANG Bei;ZHOU Hongli(College of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022

出　　处：《食品工业科技》2021年第18期183-189,共7页Science and Technology of Food Industry

基　　金：吉林省化工学院科科学技术研究项目(2020-09,2020-006);吉林省教育厅科学研究项目(JJKH20210239KJ)。

摘　　要：为进一步开发托盘根的功能性成分和药用价值。本研究以托盘的干燥根为试材,通过考察提取时间、乙醇体积分数、液料比等因素对托盘根总皂苷得率的影响,选用响应面法(RSM)探究托盘根总皂苷的最佳提取工艺,且研究了托盘根总皂苷提取物的体外抗氧化能力。结果表明:托盘根总皂苷的最优提取条件为:提取时间2 h,乙醇体积分数71%,液料比28:1 mL/g,在该条件下测得托盘根总皂苷的得率为3.37%,与预测值3.49%相近。托盘根总皂苷提取物对DPPH·、ABTS^(+)·、O_(2)^(-)·清除率的IC_(50)值分别为0.0782、0.2770和0.9954 mg/mL。因此,RSM优化托盘根总皂苷提取工艺有效可靠,稳定可行,本文为托盘根总皂苷作为天然抗氧化剂的开发与利用提供科学依据。In this study,the extraction process of total saponins from Rubus crataegifolius Bunge.roots was investigated and optimized by response surface method.The effects of ethanol concentration,extraction time and solid-liquid ratio on the extraction rate of total saponins from pallets were investigated.The results showed that the optimal extraction conditions of the total saponins from the pallets were:extraction time 2 h,ethanol concentration 71%,liquid-solid ratio 28:1 mL/g,the predicted value was 3.49%.Under these conditions,the extraction rate of total saponins from R.crataegiolius Bunge.roots was 3.366%.The IC_(50)(mg/mL)values of total saponins extract from R.crataegifolius Bunge.roots against DPPH·,ABTS^(+)·and superoxide anion free radicals were 0.0782,0.277 and 0.9954 mg/mL,respectively.Therefore,the response surface method to optimize the extraction process of total saponins from R.crataegifolius Bunge.roots was effective,reliable,stable and feasible,and this article would provide scientific basis for the development and utilization of total saponins from pallets as natural antioxidants.

关 键 词：托盘根 总皂苷 响应面设计 提取工艺 体外抗氧化性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]