超高效合相色谱法测定咖啡因含量实验设计  

Design of Experiment for Determination of Caffeine Content by Ultra Performance Convergence Chromatography

作　　者：谢一凡 姚莉韵 严忠红[2] 蔡玉兴[1] 金玉杰 李宁 Yifan Xie;Liyun Yao;Zhonghong Yan;Yuxing Cai;Yujie Jin;Ning Li(Basic Medical Experimental Teaching Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China;Medicinal Chemistry and Bioinformatics Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China)

机构地区：[1]上海交通大学医学院,基础医学实验教学中心,上海200025 [2]上海交通大学医学院,药物化学与生物信息学中心,上海200025

出　　处：《大学化学》2021年第9期145-149,共5页University Chemistry

基　　金：上海交通大学医学院课程建设基金项目(BJ13000180095)。

摘　　要：设计了一个仪器分析实验,利用超高效合相色谱仪(Ultra Performance Convergence Chromatography,UPCC或UPC^(2))测定饮料中咖啡因的含量。该实验选取可乐、绿茶、乌龙茶三种饮料,样品用甲醇萃取,萃取液无需旋蒸,直接稀释定容后进样,测定其中咖啡因的含量。通过该实验学生不仅能巩固称量、定容、移液等基本操作,还能掌握UPC^(2)的基本原理、操作及应用色谱软件处理数据的技能。本实验将新型色谱仪UPC^(2)应用于基础实验教学,可以拓展学生的科学视野,有利于培养学生的创新精神和实践能力。An experiment of instrumental analysis for determination of caffeine content in beverages by Ultra Performance Convergence Chromatography(UPC^(2))was designed.The caffeine in three beverages including coke,green tea and oolong tea was extracted and diluted by methanol.Then caffeine concentrations in beverages were measured by UPC^(2).Through this experiment,students could not only consolidate several laboratory skills such as weighing,constant volume and liquid transfer,but also master the basic theory,operation and data processing of UPC^(2).This experiment integrates the new chromatography into basic laboratory teaching,which can broaden students’scientific horizons.It is conductive to cultivating students’innovative spirit and practical ability.

关 键 词：超高效合相色谱 咖啡因 含量测定 实验教学 

分 类 号：G64[文化科学—高等教育学] O657.72[文化科学—教育学]