2’-岩藻糖基乳糖生物合成专利技术研究进展  

Review on Patented Techniques for 2’-Fucosyllactose Biosynthesis

作　　者：李云飞 栾庆民[1,2] 刘峰[1] 孙桂莲 张莉[1] 李珍珍 李克文[1,2] LI Yunfei;LUAN Qingmin;LIU Feng;SUN Guilian;ZHANG Li;LI Zhenzhen;LI Kewen(Baolingbao Biotechnology Co.Ltd.,Yucheng 251200,China;Shandong Provincial Key Laboratory of Extraction and Application Technology for Functional Glycomics,Yucheng 251200,China;School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]保龄宝生物股份有限公司,山东禹城251200 [2]山东省功能糖提取与应用技术重点实验室,山东禹城251200 [3]上海交通大学农业与生物学院,上海200240

出　　处：《乳业科学与技术》2021年第5期51-57,共7页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：2019年泰山产业领军人才(传统产业创新类)项目;2020年度山东省重点研发计划项目(2020CXGC010601)。

摘　　要：生物发酵法生产2’-岩藻糖基乳糖(2’-fucosyllactose,2’-FL),首要条件是构建出安全、高效的生产菌株。本文分析近年申请我国生物合成2’-FL相关专利的数量和技术内容,发现80%以上的专利内容围绕重组菌的构建,通过优化代谢途径、过表达2’-FL合成中相关酶的基因,寻找更安全、高效的宿主菌。然而,在我国的专利技术中,基于中试规模及以上的研究比较薄弱,实用型和面向产业化的专利技术几乎空白,这将是制约我国工业化生物合成2’-FL的瓶颈。Constructing an efficient and safe producer strain is a prerequisite for biosynthetic production of 2’-fucosyllactose.The number and technical content of recent patents on 2’-fucosyllactose biosynthesis applied for in China are reviewed in this paper,indicating that over 80 percent of the patents focused on the construction of recombinant strains by optimizing the metabolic pathways,overexpressing 2’-fucosyllactose biosynthesis-related enzyme genes,and developing more safe and efficient host strains.However,after a review of China’s patented technologies in this regard,we found that research on the biosynthetic production of 2’-fucosyllactose in a pilot scale or above is weak,and there is a lacuna in utility and industrially oriented patented technologies,which will bottleneck the 2’-fucosyllactose biosynthesis industry in China.

关 键 词：2’-岩藻糖基乳糖 重组菌 基因编辑 发酵 专利 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]