沿面放电等离子体对苹果汁中耐高渗酵母的杀灭效果  

Inactivation of Zygosaccharomyces rouxii in Apple Juice by Surface Discharge Plasma

作　　者：张文乐 岳田利 周嘉佳 朱璇 王英 ZHANG Wenle;YUE Tianli;ZHOU Jiajia;ZHU Xuan;WANG Ying(College of Food Science and Pharmacy,Xinjiang Agricultural University,Ürümqi 830052,China;College of Food Science and Technology,Northwest University,Xi’an 710069,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]西北大学食品科学与工程学院,陕西西安710069

出　　处：《食品科学》2021年第19期164-169,共6页Food Science

基　　金：新疆维吾尔自治区高校科研项目(XJEDU2020Y015)。

摘　　要：目的:研究沿面放电等离子体对苹果汁中耐高渗酵母(Zygosaccharomyces rouxii)的杀灭效果及其对苹果汁品质的影响。方法:以耐高渗酵母LB为目标菌株,首先利用不同电压和不同气体流速沿面放电等离子体处理目标菌株,通过微生物技术分析沿面放电等离子体对目标菌株的杀灭效果,并观察处理过程对细胞膜的破坏作用;其次,评估沿面放电等离子体处理对苹果汁理化指标及色泽和挥发性化合物的影响。结果表明:在21 kV等离子体处理30 min后,500 mL苹果汁中的耐高渗酵母数量降低了5.60个对数,并且等离子体处理会造成细胞膜损伤,从而导致细胞死亡。沿面放电等离子体处理对苹果汁理化指标和挥发性化合物总体没有显著影响(P>0.05),但处理前后苹果汁颜色参数变化显著(P<0.05)。结论:沿面放电等离子体对苹果汁中耐高渗酵母具有显著的杀灭效果,研究结果可为该技术应用于食品中微生物的杀灭提供理论依据。Purpose:To study the killing effect of surface discharge plasma on the osmophilic yeast Zygosaccharomyces rouxii in apple juice and its influence on the quality of apple juice.Methods:Surface discharge plasma was applied under different conditions of voltage and air flow rate.The damage caused to the cell membrane by it was observed and its influence on physicochemical indexes,color and volatile compounds of apple juice were evaluated.Results:After 30 min of plasma processing at 21 kV,the population of the osmophilic yeast in 500 mL of apple juice decreased by 5.60 logarithms,and the plasma processing caused severe cell membrane damage,leading to cell death.Surface discharge plasma processing had no significant effect on the physicochemical characteristics or volatile compounds of apple juice(P>0.05),while the color parameters were significantly changed by it(P<0.05).Conclusion:Surface discharge plasma had a potent bactericidal effect on the osmophilic yeast in apple juice,which will provide a theoretical basis for applying this technique to the inactivation of spoilage microorganisms in foods.

关 键 词：沿面放电等离子体 杀灭效果 耐高渗酵母 苹果汁 

分 类 号：TS255.44[轻工技术与工程—农产品加工及贮藏工程]