方竹叶黄酮提取工艺优化及其抗氧化能力研究  

Optimization of Extraction Process and Antioxidant Activity of Flavonoids from Chimonobambusa quadrangularis(Fenzi)Makino Leaves

作　　者：黄珊 刘嘉[1] 李贵华 刘永翔[1,3] 卢扬 刘辉[1] 黄毕应[2] 李俊 HUANG Shan;LIU Jia;LI Gui-hua;LIU Yong-xiang;LU Yang;LIU Hui;HUANG Bi-ying;LI Jun(Food Processing Institute of Guizhou Academy of Agricultural Sciences,Guiyang 550006,Guizhou,China;Niuchang Town People's Government of Fuquan City,Qiannan 550508,Guizhou,China;Guizhou Key Laboratory of Biotechnology,Guiyang 550006,Guizhou,China)

机构地区：[1]贵州省农业科学院食品加工研究所,贵州贵阳550006 [2]贵州省福泉市牛场镇人民政府,贵州黔南550508 [3]贵州省生物技术重点实验室,贵州贵阳550006

出　　处：《食品研究与开发》2021年第19期109-117,共9页Food Research and Development

基　　金：贵州省农业科学院青年基金(黔农科院青年科技基金[2020]17号);竹类病虫害防控与资源开发四川省重点实验室科研项目(ZLKF19-10)。

摘　　要：以方竹叶为原料,对比乙醇提取、纤维素酶提取、超声辅助乙醇提取、振荡辅助乙醇提取4种提取方式对方竹叶黄酮提取率的影响,通过响应面优化试验确定最优的方竹叶黄酮提取工艺,同时对4种提取方式获得的方竹叶黄酮体外抗氧化能力进行评价。结果表明,超声辅助乙醇提取的黄酮提取率最高,达到(4.06±0.06)%。优化得到方竹叶黄酮最优提取工艺为料液比1∶25(g/mL)、乙醇浓度80%、超声时间112 min、超声温度57℃、超声功率85 W。随着方竹叶黄酮质量浓度增加,不同提取方法所得黄酮的DPPH自由基清除率和Fe^(3+)还原能力均逐渐增强。超声辅助乙醇提取法所得黄酮的抗氧化能力最强。Chimonobambusa quadrangularis(Fenzi)Makino leaves were chosen as raw material.The four extraction methods,included ethanol extraction,cellulase extraction,ultrasound assisted ethanol extraction and vibration assisted ethanol extraction were evaluated by compared the extraction rate of flavonoids,and the optimal extraction process of flavonoids from Chimonobambusa quadrangularis(Fenzi)Makino leaves was obtained by the response surface optimization experiment.At the same time,the anti-oxidant ability of the extracts of Chimonobambusa quadrangularis(Fenzi)Makino leaves obtained by the four extraction methods was evaluated.The results showed that the flavonoids extracted with ultrasonic assisted ethanol had a high extraction rate up to(4.06±0.06)%.The optimum extraction conditions were as follows:the ratio of solid to liquid was 1∶25(g/mL),the ethanol concentration was 80%,ultrasonic time was 112 min,ultrasonic temperature was 57℃,ultrasonic power was 85 W.With the increase in the mass concentration of the flavonids of Chimonobambusa quadrangularis(Fenzi)Makino leaves,the DPPH free radical scavenging ability and Fe^(3+)reduction ability of the flavonids by different extraction methods were gradually enhanced.The antioxidant capacity of the flavonids by ultrasonic assisted ethanol extraction were the strongest.

关 键 词：方竹叶 黄酮 响应面法 提取工艺 超声 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]