组胺胶体金免疫快速检测试纸条研制及其在水产品检测中的应用  

Development of histamine colloidal gold immune rapid detection test strip and its application in aquatic products

作　　者：王炳志 骆和东 叶雅真 严义勇 袁克湖 杨星星 许稳健 颜文豪 WANG Bing-Zhi;LUO He-Dong;YE Ya-Zhen;YAN Yi-Yong;YUAN Ke-Hu;YANG Xing-Xing;XU Wen-Jian;YAN Wen-Hao(Shenzhen Bioeasy Biotechnology Co.,Ltd.,Shenzhen 518101,China;Xiamen Institute for Food and Drug Quality Control,Xiamen 361012,China)

机构地区：[1]深圳市易瑞生物技术股份有限公司,深圳518101 [2]厦门市食品药品质量检验研究院,厦门361012

出　　处：《食品安全质量检测学报》2021年第18期7206-7213,共8页Journal of Food Safety and Quality

基　　金：国家市场监督管理总局项目(KJ202102);深圳市科创委项目(JSGG20180508152235868);广东省科技厅项目(2014ZT05S123)。

摘　　要：目的研制基于胶体金免疫层析法的组胺快速检测试纸条,并探究其在水产品中的应用。方法以组胺为原料,采用琥珀酸酐对其衍生化合成半抗原;活泼酯法将半抗原与白蛋白偶联得到人工抗原;用人工抗原免疫小鼠获得单克隆抗体。采用上述原料制备出本研究试纸条,依据国家《食品快速检测方法评价技术规范》进行评价。结果所研制试纸条对水产品中组胺检出限为高组胺鱼类400mg/kg、一般海水鱼类及虾200 mg/kg。可特异性地检测残留组胺,而对其他与组胺结构或功能相似的生物胺无交叉反应。结论本研究提供的试纸条操作简便,具有较高的灵敏度和特异性,可广泛应用于水产品中组胺的现场筛查和快速检测。Objective To establish a rapid test strip for histamine detection based on colloidal gold immunochromatography, and explore its application in aquatic products. Methods Histamine was used as starting material and succinic anhydride was used to derivatize it to synthesize the hapten;activated ester method was used to couple the hapten with albumin to obtain artificial antigen;mice were immunized with artificial antigen to obtain monoclonal antibody. The test strip for this study was prepared as described above, the strip was evaluated according to the national "Food rapid detection method evaluation technical specification". Results The detection limits of the strip for high histamine fish and common fish and shrimp were 400 and 200 mg/kg respectively. The residue of histamine could be specifically detected with no obvious cross reaction to other biogenic amines with similar structure or function to histamine. Conclusion The test strip provided in this study is easy to operate, with high sensitivity and specificity, and can be widely used in the field screening and rapid detection of histamine in aquatic products.

关 键 词：组胺 胶体金免疫层析法 水产品 快速检测 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]