原子荧光紧密内插法快速测定莲藕中砷的生物吸收比  

Rapid determination of biological absorption ratio of arsenic in lotus root by atomic fluorescence close interpolation

作　　者：高向阳[1,2] 王彦花 郭楠楠 GAO Xiang-Yang;WANG Yan-Hua;GUO Nan-Nan(College of Food Science and Engineering,Zhengzhou University of Science and Technology,Zhengzhou 450064,China;Zhengzhou City Food Safety Rapid Test Key Laboratory,Zhengzhou 450064,China)

机构地区：[1]郑州科技学院食品科学与工程学院,郑州450064 [2]郑州市食品安全快速检测重点实验室,郑州450064

出　　处：《食品安全质量检测学报》2021年第18期7281-7285,共5页Journal of Food Safety and Quality

摘　　要：目的建立原子荧光法快速测定莲藕中微量砷和生物吸收比。方法按照GB 5009.11—2014《食品安全国家标准食品中总砷及无机砷的测定》中的原子荧光法对照测定,用微波辅助消解样品,用紧密内插法测定莲藕各组织和土壤中的砷。结果方法线性关系好(r^(2)=0.9997),检出限为0.00732ng/mL,定量限为0.0244ng/mL。平均回收率为99.80%~101.19%,相对标准偏差小于1%。各组织砷的生物吸收比为22.03%~79.54%,与国标法相比,通过显著性检验,方法之间没有显著性差异(P>0.05)。结论该法不需要绘制标准曲线和测定空白,快速简便、计算简单、成本低廉,拓展了原子荧光分析技术的应用范围,具有一定的创新性和推广价值。Objective To establish an anatomic fluorescence method for rapid determination of trace arsenic and biological absorption ratio in lotus root. Methods According to GB 5009.11—2014 National food safety standard-Determination of total arsenic and inorganic arsenic in food, the samples were digested by microwave-assisted digestion, and the arsenic in lotus root tissues and soil was determined by close interpolation method. Results The linear relationship of the method was good(r^(2)=0.9997), the detection limit was 0.00732 ng/mL and the quantitative limit was 0.0244 ng/mL. The average recovery rates were 99.80%-101.19%, and the relative standard deviations were less than 1%. The biological absorption ratioes of arsenic in each tissue were 22.03%-79.54%. Compared with the national standard method, there was no significant difference between the methods through the significance test(P>0.05). Conclusion This method does not need to draw the standard curve and determine the blank, it is fast and simple, the calculation is simple, and the cost is low. It expands the application scope of atomic fluorescence analysis technology, and has a certain innovation and promotion value.

关 键 词：紧密内插法 原子荧光法 莲藕 砷 生物吸收比 

分 类 号：O657.31[理学—分析化学] TS254.58[理学—化学]