Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease  

作　　者：Jia-Nan Yan Hai-Ying Zhang Jun-Rui Li Ying Chen Yong-Cheng Jiang Jia-Bing Shen Kai-Fu Ke Xiao-Su Gu 

机构地区：[1]Department of Neurology,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China [2]Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China [3]Department of Clinical Medicine,The First Clinical Medical College of Xuzhou Medical University,Xuzhou,Jiangsu Province,China [4]Department of Neurology and Suzhou Clinical Research Center of Neurological Disease,The Second Afflicted Hospital of Soochow University,Suzhou,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2022年第6期1357-1363,共7页中国神经再生研究（英文版）

基　　金：Technology Project of Nantong of China,Nos.JC2020052(to XSG),JCZ19087(to XSG);the National Natural Science Foundation of China,Nos.81873742(to KFK),81901195(to JBS),81502867(to TX),82073627(to TX).

摘　　要：Autophagy has been shown to play an important role in Parkinson’s disease.We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy.In this study,6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells(SKP-SCs).The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine,reduced excessive autophagy,increased tyrosine hydroxylase expression,reducedα-synuclein expression,reduced the autophagosome number,and activated the PI3K/AKT/mTOR pathway.Autophagy activator rapamycin inhibited the effects of SKP-SCs,and autophagy inhibitor 3-methyladenine had the opposite effect.These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy,thereby exhibiting a neuroprotective effect in a cellular model of Parkinson’s disease.This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University(approval No.S20181009-205)on October 9,2018.

关 键 词：alpha-synuclein AUTOPHAGOSOMES AUTOPHAGY neural regeneration NEUROPROTECTION Parkinson’s disease PI3K/AKT/mTOR pathway skin-derived precursor Schwann cells 

分 类 号：R446.1[医药卫生—诊断学] R741[医药卫生—临床医学]