蚓粪对大蒜中蒜氨酸和大蒜素含量及大蒜精油抗菌活性的影响  

Effects of vermicompost on alliin and allicin contents and antibacterial activity of garlic oil in garlic

作　　者：叶嘉 张浩 郭海燕 宁辉 YE Jia;ZHANG Hao;GUO Haiyan;NING Hui(School of Life Science and Engineering,Handan College,Handan 056005,Hebei,China;Wild Plant Resources Research Centre in Taihang Mountain of Southern Hebei,Handan 056005,Hebei,China)

机构地区：[1]邯郸学院生命科学与工程学院,河北邯郸056005 [2]河北省高校冀南太行山区野生资源植物应用技术研发中心,河北邯郸056005

出　　处：《中国瓜菜》2021年第10期98-103,共6页China Cucurbits And Vegetables

基　　金：河北省科技支撑计划项目(18226435);邯郸市科学技术研究与发展计划项目(1727201061-2);河北省高等学校科学技术研究项目(ZD2017302,ZD2019315);邯郸学院校级重点项目(2018104,XZ2019310)。

摘　　要：以大蒜为材料,测定不同处理下蒜氨酸和大蒜素含量并检测大蒜精油对金黄色葡萄球菌和沙门氏菌的抑制效果,为开发大蒜天然抑菌药物和高效防腐保鲜剂提供参考。结果表明,施用蚓粪和化肥的大蒜蒜氨酸含量(w,后同)分别为44.05 mg·g^(-1)和33.86 mg·g^(-1),大蒜素含量分别为0.33 mg·g^(-1)和0.13 mg·g^(-1),施用蚓粪的大蒜蒜氨酸和大蒜素含量均显著高于施用化肥处理;施用蚓粪的大蒜精油得率显著高于化肥处理;在1.25~40.0 mg·mL-1质量浓度内,2种施肥处理大蒜提取得到的精油对金黄色葡萄球菌和沙门氏菌的最小抑菌质量浓度均为2.5 mg·mL-1,并表现出一定的量效关系;同一浓度处理下,蚓粪处理的大蒜精油对金黄色葡萄球菌和沙门氏菌的抑制作用优于化肥处理。In order to provided reference data for the development of natural antibacterial drugs and high-efficiency preservatives, the contents of alliin, allicin and the inhibitory effect of garlic oil on Staphylococcus aureus and Salmonella typhimurium were detected. The results showed that the contents of alliin were 44.05 mg·g^(-1) and 33.86 mg·g^(-1), after treated by vermicompost and chemical fertilizer respectively, and allicin were 0.33 mg · g^(-1) and 0.13 mg · g^(-1), respectively. The contents of alliin and allicin treated with vermicompost were significantly higher than those of fertilizer treatment. The yield of garlic essential oil in vermicompost substrate was significantly higher than that in fertilizer substrate. The minimum inhibitory concentration of garlic oil contained in vermicompost and chemical fertilizer substrate to Staphylococcus aureus and Salmonella typhimurium was 2.5 mg·mL-1 in the range of 1.25-40.0 mg·mL-1, and it was showed a certain dose effect relationship. With the increase of garlic essential oil concentration, the diameter of bacteriostatic circle of Staphylococcus aureus and Salmonella typhimurium increased gradually. At the same concentration, the inhibition of garlic essential oil in vermicompost matrix on Staphylococcus aureus and Salmonella typhimurium was better than that in fertilizer matrix.

关 键 词：大蒜 高压液相色谱法 蒜氨酸 大蒜素 大蒜精油 抑菌 

分 类 号：S633.4[农业科学—蔬菜学]