一株产果香型真菌的筛选、鉴定及在烟草增香中的应用  

Screening and Identification of a Fungus with a Sweet Fruit Aroma and Its Application in Flavoured Tobacco

作　　者：张鹏[1] 李炜 师超 高加明 任杰[1] 申国明[1] 张忠锋[1] ZHANG Peng;LI Wei;SHI Chao;GAO Jiaming;REN Jie;SHEN Guoming;ZHANG Zhongfeng(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Shanghai Tobacco Group Beijing Cigarette Factory Co.,Ltd.Beijing 101121,China;Lichuan Branch of Enshi Tobacco Company,Lichuan,Hubei 445400,China;Hubei Provincial Tobacco Company of China National Tobacco Corporation,Wuhan 430000,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]上海烟草集团北京卷烟厂有限公司,北京101121 [3]湖北省恩施州烟草公司利川市烟叶分公司,湖北利川445400 [4]中国烟草总公司湖北省公司,武汉430000

出　　处：《中国烟草科学》2021年第5期95-101,共7页Chinese Tobacco Science

基　　金：上海烟草集团北京卷烟厂有限公司科技项目(TP2016-C6,TP2021-T2);湖北省烟草公司科技项目(027Y2018-013)。

摘　　要：烟叶香气不足一直制约着烟叶品质及其工业可用性。为开发新的烟草香料,本研究从湖北省利川市烟草种植区的烟叶中筛选到产果香型菌株R59,经鉴定为产黑色素短梗霉Aureobasidium melanogenum。发酵后,该菌株能够产生11种香气化学物质,其中果香型的苯乙醇、苯乙醛和醋酸异戊酯的浓度均超过100μg/mL。对发酵培养基进行优化,发现以30 g/L的葡萄糖作为碳源,15 g/L玉米浆作为氮源,培养基pH为5.0条件下培养R59菌株,可有效提高菌株中苯乙醛和醋酸异戊酯的产量。喷洒10μL/g R59菌株发酵液可有效改善烟丝的协调性、香气和余味。R59菌株产香发酵成本低,添加工艺简单,可以作为一种提高烟草品质的香料来源。Insufficient aroma of tobacco leaves has always been a major factor limiting the quality of tobacco leaves and their industrial availability.In order to develop a new source of spices,the R59 strain with a sweet fruit aroma was isolated from healthy leaves of tobacco collected from Lichuan,Hubei province.This strain was identified as Aureobasidium melanogenum.In this study,eleven aroma components were detected in the fermentation broth.Among them,phenylethyl alcohol,phenylacetaldehyde,and isoamyl acetate,which possessed fruity arama,were produced more than 100μg/mL.After fermentation medium optimization,the production of phenylacetaldehyde and isoamyl acetate by the R59 strain was increased significantly when cultured in 30 g/L glucose,15 g/L corn steep liquor at pH 5.0.Spraying of the fermentation broth onto cut tobacco at a dosage of 10μL/g could effectively improve its coordination,aroma and aftertaste.Due to its low fermentation cost and simple addition process,the R59 strain can be used as an aroma source to significantly improve the quality of cigarette products.

关 键 词：果香型真菌 烟草 增香 感官评价 

分 类 号：TS452[农业科学—烟草工业]