野生牛肝菌内生真菌分离鉴定及其生物学特性  

Isolation,Identification and Biological Characteristics of Endophytic Fungi from Wild Boletus

作　　者：徐伟[1] 张雪 王植朔 谢红瑶 吴凡 邵百琪 XU Wei;ZHANG Xue;WANG Zhishuo;XIE Hongyao;WU Fan;SHAO Baiqi(School of Food Engineering,Harbin University of Commerce,Harbin 150028,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028

出　　处：《食品工业科技》2021年第21期118-124,共7页Science and Technology of Food Industry

摘　　要：采用组织分离法从黄皮疣柄牛肝菌、小美牛肝菌、双色牛肝菌的子实体中,分离纯化获得4株内生真菌,经菌落、菌丝、孢子形态学观察及rDNA ITS分子生物学鉴定,结果显示:菌株LCTG-12为盖姆斯木霉(Trichoderma gamsii)、LCCK-6为克鲁伊假丝酵母(Candida kruisii)、BSHC-18为黄瘤孢菌(Hypomyces chrysospermus)、BBFO-10为尖孢镰刀菌(Fusarium oxysprum)。对4株内生真菌生物学特性进行研究,其中盖姆斯木霉菌丝生长速度较快,在CPDA培养基上,25℃培养5 d菌丝长满平板;液态培养时,该菌株在25℃、摇床转速160 r/min、pH5.5、8 d,可获得菌丝干基生物量4.13 g/L。平板对峙实验结果:盖姆斯木霉LCTG-12对黄瘤孢菌BSHC-18和尖孢镰刀菌BBFO-10菌丝生长均有拮抗作用,并且,黄瘤孢菌BSHC-18和尖孢镰刀菌BBFO-10两者之间也具有拮抗作用,表明牛肝菌内生真菌中盖姆斯木霉对腐败菌具有拮抗作用,可为生物防腐提供优良的菌株。Four strains of endophytic fungi were isolated and purified from wild bodies of Leccinum crocipodium,Boletus speciosus,and Boletus bicolor by tissue separation method.Microscopic observation of its colonies,hyphae,and conidia morphology,and molecular biological identification of rDNA ITS sequence results showed that:Strain LCTG-12 was Trichoderma gamsii,LCCK-6 was Candida kruisii,BSHC-18 was Hypomyces chrysospermus,BBFO-10 was Fusarium oxysprum.The biological characteristics of four endophytic fungi studied,among them,the mycelium of Trichoderma gamsii growed faster,cultured on CPDA medium at 25℃for 5 days,mycelium could cover the entire medium.In liquid culture,the strain could obtain 4.13 g/L of dry biomass of mycelium at 25°C,shaker speed 160 r/min,pH5.5,and 8 days.The results of the flat panel stand-off experiment:LCTG-12 Trichoderma gamsii had an antagonistic effect on the growth of BSHC-18 Hypomyces chrysospermus and BBFO-10 Fusarium oxysprum,at the same time,BSHC-18 Hypomyces chrysospermus and BBFO-10 Fusarium oxysporum had an antagonistic effect.Therefore,the endophytic fungi Trichoderma gamsii of boletus had an antagonistic effect on spoilage fungi and may be an excellent strain for biological control.

关 键 词：野生牛肝菌 组织分离法 内生真菌 ITS 分子鉴定 

分 类 号：TS201.3[轻工技术与工程—食品科学]