拮抗铜绿假单胞菌的乳酸菌筛选及机制研究  

Screening of lactic acid bacteria against Pseudomonas aeruginosa and preliminary study on its mechanism

作　　者：李建周 肖尧婷[3] 李红霄 陈晓华 李安平 LI Jian-zhou;XIAO Yao-ting;LI Hong-xiao;CHEN Xiao-hua;LI An-ping(College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004,China;College of Life Sciences and Environment,Hengyang Normal University,Hengyang,Hunan 421008,China;Nanyue College of Hengyang Normal University,Hengyang,Hunan 421002,China;Hunan Key Laboratory for Conservation and Utilization of Biological Resources of Nanyue Mountainous Region,Hengyang Normal University,Hengyang,Hunan 421008,China)

机构地区：[1]中南林业科技大学食品学院,湖南长沙410004 [2]衡阳师范学院南岳学院,湖南衡阳421002 [3]衡阳师范学院生命科学与环境学院,湖南衡阳421008 [4]衡阳师范学院南岳山区生物资源保护与利用湖南省重点实验室,湖南衡阳421008

出　　处：《食品与机械》2021年第10期6-11,149,共7页Food and Machinery

基　　金：湖南省自然科学基金(编号:2019JJ50014);国家自然基金青年基金(编号:31601444);衡阳师范学院大学生创新创业训练计划项目(编号:cxcy1934);衡阳师范学院大学生课外学术科技作品竞赛(编号:校科字[2019]2号)。

摘　　要：目的:以铜绿假单胞菌(Pseudomonas aeruginosa,PA)为指示菌,基于群体感应系统筛选拮抗PA作用的乳酸菌(Lactic acid bacteria,LAB)并探讨拮抗机制。方法:通过牛津杯法筛选抑制PA生长的LAB,测定LAB的AI-2表达量,LAB抑制PA生物膜形成力、绿脓菌素表达量及LAB无菌上清液与PA共培养时AI-2信号分子的表达量,并利用皮尔森相关性分析探讨LAB拮抗PA机制。结果:格氏乳杆菌LGchen和唾液乳杆菌S-5-6的整体抗菌效果比较强,抑菌圈达到20 mm以上,AI-2相对荧光值达到1.2以上,抑制PA的绿脓菌素表达率达50%以上,抑制PA生物膜形成率达70%左右。LAB抑制PA生长的能力与LAB的表达AI-2的能力、抑制PA的生物膜形成能力呈正相关,与PA的绿脓菌素表达呈负相关。结论:LAB可以通过提高AI-2表达,抑制PA生物膜形成和绿脓菌素表达来抑制PA的生长。Objective:In this study,Pseudomonas aeruginosa(PA)was used as the indicator bacteria,and based on the quorum sensing system,the lactic acid bacteria(LAB)that antagonized the effect of PA were screened and the antagonistic mechanism was preliminarily discussed.Methods:The mechanism of LAB antagonizing PA was explored by the tests of Oxford Cup Method screening LAB to inhibit the growth of PA,AI-2 expression of LAB,biofilm formation ability of PA,pyocyanin expression and the AI-2 expression of PA with LAB sterile supernatants,and the analysis of Pearson correlation.Results:The results showed that Lactobacillus gasseri LGchen and Lactobacillus salivarius S-5-6 have stronger abilities of antagonizing PA growth.The inhibition zone was more than 20 mm,and the relative fluorescence value of AI-2 was more than 1.2,and the inhibition rate of PA pyocyanin expression was above 50%,and the inhibition rate of PA biofilm formation was about 70%.The ability of LAB to inhibit the growth of PA was positively correlated with the ability of LAB to express AI-2 and the inhibition biofilm formation ability of PA,and this was negatively correlated with the pyocyanin expression of PA.Conclusion:LAB can inhibit the growth of PA by increasing the expression of AI-2,inhibiting PA biofilm formation and pyocyanin expression.

关 键 词：乳酸菌 铜绿假单胞菌 群体感应 自诱导分子AI-2 

分 类 号：TS201.3[轻工技术与工程—食品科学]