L-阿拉伯糖大鼠28 d重复剂量经口毒性研究  

Study on the L-arabinose 28 d repeated dose oral toxicity in rats

作　　者：陈耿[1] 刘协[1] 施伟庆[1] 陆罗定[1] 吴俊[1] 杨明晶[1] 吕中明[1] 卞倩[1] 奚清丽[1] CHEN Geng;LIU Xie;SHI Wei-Qing;LU Luo-Ding;WU Jun;YANG Ming-Jing;LV Zhong-Ming;BIAN Qian;XI Qing-Li(Institute of Toxicology and Risk Assessment,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 210009,China)

机构地区：[1]江苏省疾病预防控制中心毒理与风险评估研究所,南京210009

出　　处：《食品安全质量检测学报》2021年第19期7778-7786,共9页Journal of Food Safety and Quality

摘　　要：目的研究L-阿拉伯糖对大鼠的亚急性经口毒性。方法根据GB 15193.22—2014《食品安全国家标准28天经口毒性试验》,选取无特定病原体(specific pathogen free,SPF)级健康SD (Sprague-Dawley)大鼠,依体质量随机分成4组,将L-阿拉伯糖分别按照0.00、2.50、5.00、10.00 g/kg掺入基础饲料,连续饲喂28d,同时加设对照组和高剂量组2个卫星组,恢复期为14 d。主试验和恢复期结束时,称量空腹体质量和主要脏器湿重,采集血样,进行血液学、血清生化和组织病理学检测。结果与对照组相比,L-阿拉伯糖可有效降低雌、雄大鼠的摄食量和体质量,且正常饮食后可恢复正常;未见L-阿拉伯糖对大鼠一般状况、眼、食物利用率、尿常规、血液学、血生化、脏器湿重、脏器系数、大体解剖及组织病理学产生有毒理学意义的影响。结论在本研究条件下,L-阿拉伯糖对雌、雄大鼠28 d经口毒性无可见有害作用剂量(no observed adverse effect level,NOAEL)值分别为13.67和13.92 g/kg(分别相当于15 g/d推荐摄入量的55和56倍)。Objective To evaluate sub-acute oral toxicity of L-arabinose in rats.Methods According to GB15193.22—2014 National food safety standard-Repeated dose 28-day oral toxicity study,specific pathogen free(SPF) grade healthy Sprague-Dawley(SD) rats were selected and divided randomly based on their body weights into 4 groups,L-arabinose were administered via diet at dose levels of 0.00,2.50,5.00 and 10.00 g/kg for 28-day,satellite groups of control group and high dose group were added,and the recovery period was 14 d.At the end of the treatment and recovery periods,empty body and the main organs were weighed and blood samples were collected for the hematology,serum biochemistry and histopathology inspection.Results Compare with the control group,L-arabinose could significant decrease the body weights and food intakes of male and female rats,and the indicators could recovered after treatment-free;there were no L-arabinose-related toxicological implications on clinical observations,eyes,food utilization,urine routine,hematology,blood chemistries,organ weights,organ/body weight ratios,gross necropsies as well as histopathology in rats.Conclusion Under the conditions of this study,the no observed adverse effect levels(NOAEL) for L-arabinose on male and female rats are determined as 13.67,13.92 g/kg respectively(which are 55 and 56 times of 15 g/d for recommended dose in adults) when administered via diet for 28 consecutive days.

关 键 词：L-阿拉伯糖 大鼠 亚急性 毒性 

分 类 号：TS201.6[轻工技术与工程—食品科学]