鲮鱼糜致腐微生物分离及其复合生物防腐剂研究  

Study on Spoilage Microorganisms and Complex Bio-preservatives in Mud Carp(Cirrhinus molitorella)Surimi

作　　者：柏建玲 王惠惠 蔡淑珍 莫树平 杨小鹃 何莹龙 苏悦 吴清平 BAI Jian-ling;WANG Hui-hui;CAI Shu-zhen;MO Shu-ping;YANG Xiao-juan;HE Ying-long;SU Yue;WU Qing-ping(Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,InstituteofMicrobiology Guangdong Academy of Sciences,Guangzhou 510070,Guangdong,China)

机构地区：[1]广东省科学院微生物研究所,华南应用微生物国家重点实验室,广东省微生物安全与健康重点实验室,广东广州510070

出　　处：《食品研究与开发》2021年第21期137-142,共6页Food Research and Development

基　　金：国家重点研发计划(2017YFC1600403);广东省科学院发展专项项目(2019GDASYL-0201001)。

摘　　要：应用ε-聚赖氨酸和螯合增效剂复合控制冷鲜鲮鱼鱼糜中的致腐微生物。采用微生物学方法从市售鲮鱼鱼糜中分离鉴定了9株致腐微生物,选择其中5株优势致腐微生物菌株和3株标准菌株进行ε-聚赖氨酸增效剂筛选,根据筛选结果配制两种ε-聚赖氨酸复合生物防腐剂,并进行复合生物防腐剂对致腐微生物的抑菌效果及其对冷鲜鲮鱼糜的防腐效果研究。结果表明,乙二胺四乙酸二钠(ethylene diamine tetraacetic acid disodium salt,EDTA-2Na)、甘氨酸、L-丙氨酸、L-苹果酸对ε-聚赖氨酸抑菌效果具有较强的增效作用,复合生物防腐剂对试验菌株均表现出较好的抑制作用,使用复合生物防腐剂的鲮鱼糜中菌落总数、挥发性盐基氮含量均明显低于对照组。ε-polylysine and chelate were combined to control the spoilage microorganisms in aftermarket mud carp(Cirrhinus molitorella)surimi.Nine strains of spoilage microorganisms were isolated from the mud carp surimi and identified using a microbiological method.Five dominant strains and three standard strains were selected for the screening ofε-polylysine synergy.According to the screening results,twoε-polylysine complex bio-preservatives were prepared,their bacteriostatic and antiseptic effect in chilled mud carp surim were studied.The results showed that ethylene diamine tetraacetic acid disodium salt(EDTA-2Na),glycine,L-alanine,and L-malic acid had a strong synergistic effect withε-polylysine.The complex bio-preservative showed a good inhibitory effect on the test strains.The total number of colonies and the content of volatile base nitrogen in mud carp surimi were significantly reduced by the complex bio-preservatives compared with those in the control group.

关 键 词：鲮鱼 致腐微生物 抑菌 增效剂 复合生物防腐剂 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]