ORAC法比较5种食源性肽的抗氧化能力  

Comparative Study on Antioxidant Activity of Five Food-derived Bioactive Peptides by ORAC Method

作　　者：韩绍聪 黄蓉萍 闫昌誉 余桂媛 李维熙 Han Shaocong;Huang Rongping;Yan Changyu;Yu Guiyuan;Li Weixi(Yunnan University of Traditional Chinese Medicine,College of Traditional Chinese Medicine,Kunming 650500;Ji’nan University,College of Pharmacy,Guangzhou 510632,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]暨南大学药学院,广东广州510632

出　　处：《广东化工》2021年第21期15-16,36,共3页Guangdong Chemical Industry

基　　金：国家自然科学基金(81560661,81960780);云南省基础研究中医联合专项(2017FF116(-015),2019FF002(-007))。

摘　　要：目的:探讨玉米肽、大米肽、小麦肽、大豆肽和核桃肽5种食源性肽的体外抗氧化活性。方法:采用氧自由基吸收能力(ORAC)法,以水溶性VE(Trolox)为定量标准,对5种食源性肽进行抗氧化活性检测。结果:玉米肽、大米肽、小麦肽、大豆肽和核桃肽的ORAC当量分别为6.004、5.405、4.469、3.253和7.527μmol Trolox/mg。结论:五种活性肽均有较好的抗氧化能力(3.2~7.5μmol Trolox/mg),其中核桃肽活性最强,其次是玉米肽、大米肽、小麦肽和大豆肽。Objective:To investigate antioxidant activities of five food-derived peptides,including corn peptide,rice peptide,wheat peptide,soybean peptide and walnut peptide.Methods:Oxygen radical absorbance capacity(ORAC)method was adopted,and the water-soluble VE(Trolox)was applied as the quantitative standard.Results:The ORAC equivalents of corn peptide,rice peptide,wheat peptide,soybean peptide and walnut peptide were 6.004,5.405,4.469,3.253 and 7.527μmol Trolox/mg,respectively.Conclusion:The five peptides have good antioxidant capacity(3.2~7.5μmol Trolox/mg).Among them,walnut peptide has the strongest activity,followed by corn peptide,rice peptide,wheat peptide and soybean peptide.

关 键 词：ORAC 食源性 活性肽 抗氧化 TROLOX 

分 类 号：TQ[化学工程]