以大鼠骨髓基质干细胞进行流式细胞术教学的效果研究  

Study on the Effect of Flow Cytometry Teaching with Rat Bone Marrow Stromal Stem Cells

作　　者：邵志华[1] 李姣[2] 许洁[2] 贾松[2] 沙继宏[1] 吕立夏[3] 徐磊[3] 李思光[1] SHAO Zhihua;LI Jiao;XU Jie;JIA Song;SHA Jihong;Lü Lixia;XU Lei;Li Siguang(Department of Cell Biology,School of Medicine,Tongji University,Shanghai 200092,China;Teaching Laboratory Center of Medicine and Life Science,School of Medicine,Tongji University,Shanghai 200092,China;Department of Biochemistry and Molecular Biology,School of Medicine,Tongji University,Shanghai 200092,China)

机构地区：[1]同济大学医学院细胞生物学系,上海200092 [2]同济大学医学院医学与生命科学实验教学中心,上海200092 [3]同济大学医学院生物化学与分子生物学系,上海200092

出　　处：《实验室研究与探索》2021年第10期189-192,共4页Research and Exploration In Laboratory

基　　金：国家自然科学基金项目(81771333);同济大学实验教改基金项目(1500104185)。

摘　　要：为了优化精品实验项目,提高实验教学水平,在原有大鼠骨髓基质干细胞培养与分化实验基础上,将干细胞培养与流式细胞术相结合,建立了以"大鼠骨髓基质干细胞为材料的流式细胞术实验"。实验采用翻转课堂教学法,注重理论知识、实验操作、软件使用和数据分析多种能力的培养。结果显示实验稳定、可行,100%的学生认为实验促进了对干细胞特性的理解,提高了学习效果。课后测试平均成绩明显高于课前,差异有统计学意义。问卷调查显示,学生已基本熟练掌握流式工作原理、操作流程、对照实验设计、软件的使用和结果分析。实验首次以干细胞为材料进行流式细胞术教学,提高了学生的流式技能,加深了学生对干细胞特征的理解,提高了学生的前沿知识水平和实验技能。In order to optimize the excellent experimental projects and improve the level of experimental teaching, we established the flow cytometry experiment with rat bone marrow stromal cells on the basis of the original experiment. The results show that the experiment is stable and feasible, and 100% of the students consider that the experiment promotes the understanding of stem cell characteristics and deepens the learning depth. The comparison between pre-and post-test shows that the average score of post-test is significantly higher than that of pre-test, the difference was statistically significant. The questionnaire shows that the students have mastered the flow working principle, operation process, control design, software use and result interpretation. For the first time, stem cells were used in experimental teaching of flow cytometry, and the experiment improved students’ skills of flow cytometry and deepened their understanding of stem cell characteristics, and improved students’ frontier knowledge and experimental skills.

关 键 词：实验教学 流式细胞术 干细胞 临床医学 

分 类 号：G642[文化科学—高等教育学]