气相色谱法测定啤酒中N-亚硝胺类化合物  

Determination of N-Nitrosamine Compounds in Beer by Gas Chromatography

作　　者：田冲[1] 葛怡聪 陈俏[1] 陈稼轩[1] 聂万丽[1] 马克西姆 TIAN Chong;GE Yicong;CHEN Qiao;CHEN Jiaxuan;NIE Wanli;MAXIM(School of Chemistry and Resources Environment,Leshan Normal University,Leshan 614000,China)

机构地区：[1]乐山师范学院,化学与资源环境学院,四川乐山614000

出　　处：《食品科技》2021年第10期294-297,共4页Food Science and Technology

基　　金：四川省科技厅基础研究项目(20YYJC0829);乐山师范学院项目(LZD026,DGZZ202018,TRCWYFZCH2019004)。

摘　　要：目的:建立啤酒中7种亚硝胺类化合物的气相检测方法。方法:分别考察不同极性毛细管柱、氯化钠加入量、二氯甲烷使用量、振摇时间等因素对提取分析效果的影响,最终确定啤酒样品加入4 g氯化钠后经3 mL二氯甲烷提取,振摇2 min,经Wax毛细管柱分离,NPD检测器检测。结果:该方法线性关系良好,回收率81.2%~87.0%,精密度1.1%,重复性3.6%,定量限0.50~1.1μg/L。结论:该方法处理过程简单,易于操作,有机试剂消耗少,适合用于啤酒中N-亚硝胺类化合物的检测。Object:To establish a method for determination of 7 kinds of N-nitrosamine compounds by GC in Beer.Methods:Sample beer was put into a 50 mL centrifuge tube with 4 g sodium sulfate added.After dissolution with manual vibrating,3 mL dichloromethane was injected to the solution,shaking and centrifugation,the bottom organic phase of sample solution was filtered before injected to the GC,the compound was carried out on Wax column.Results:Good linearity was observed in the ranges of 0~200 ng/mL and the limit of quantitation was 0.50~1.1μg/L.The recovery was 81.2%~87.0%,which precision was 1.1%,and repeatability of the method was 3.6%.Conclusion:The simplicity,sensitivity and good precision of the method made it well suitable for determination of N-nitrosamine compounds in Beer.

关 键 词：啤酒 气相色谱 N-亚硝胺 

分 类 号：TS262.57[轻工技术与工程—发酵工程]