响应面优化海洋担子菌产葡萄糖氧化酶发酵条件研究  

Optimization of glucose oxidase production from marine basidiomycetes by response surface methdology

作　　者：张庆芳[1,2] 王倩倩 迟雪梅 于爽[1,2] 陈立功 迟乃玉[1,2] 刘春莹 ZHANG Qingfang;WANG Qianqian;CHI Xuemei;YÜShuang;CHEN Ligong;CHI Naiyü;LIU Chunying(School of Life Science and Technology,Dalian University,Dalian 116622;Liaoning Provincial Marine Microbial Engineering Technology Research Center,Dalian 116622)

机构地区：[1]大连大学生命科学与技术学院,大连116622 [2]辽宁省海洋微生物工程技术研究中心,大连116622

出　　处：《中国食品添加剂》2021年第11期9-16,共8页China Food Additives

基　　金：国家高技术研究发展计划‘863计划’项目(2018YFC0311100);高层次人才创新支持计划(2018RQ13)。

摘　　要：从海泥中筛选到了一株高产低温葡萄糖氧化酶(Glucose oxidase,GOD)的担子菌(Basidioascus sp.)酵母菌株(31号),在前期单因素优化实验(单因素优化结果为:蔗糖4%、酵母粉0.3%、NaNO_(3)0.4%、无机盐添加量(KH_(2)PO_(4)0.02%、KCl 0.10%、MgSO_(4)·7H_(2)O 0.10%)、初始pH7.0、装液量125mL/250mL、接种量2%、发酵转速200r/min、发酵温度20℃,酶活力达到435.37U/mL)的基础上,对其进行PlackettBurman、Box-Behnken等相关实验,以进一步提高31号菌株产GOD酶活性,结果表明,GOD酶活性显著提高。Plackett-Burman实验确定了发酵温度、蔗糖添加量和接种量是影响31号菌株产酶的重要因素。最陡爬坡实验及响应面实验联合优化,确定了三个最显著影响因素的最佳培养条件为发酵温度26℃、蔗糖3.92%和接种量3.58%。根据实际情况,进行适当调整,确定31号菌株产GOD酶的最佳条件,在最优发酵条件下,菌株产GOD酶活力达503.22U/mL,与优化前酶活435.37U/mL相比,优化后GOD酶活提高了15.58%。Strain 31 originated from sea mud of the Bohai Sea in Dalian.In order to improve GOD enzyme activity of strain 31,based on single factor optimization in the early stage,Plackett-Burman,steepest climbing and Box-Behnken experiments were performed to select the best conditions on enzyme activity.The results showed GOD enzyme activity is significantly improved.The Plackett-Burman experiment determined that the fermentation temperature,the amount of sucrose and inoculum are important factors that affect the enzyme production of strain 31.The steepest climbing experiment and response surface experiment were combined in selecting the optimal culture conditions.The best conditions for the three most significant influencing factors were:the fermentation temperature 26℃,sucrose amount 3.92%,and the inoculum amount 3.58%.According to the actual situation,appropriate adjustments were made to select the optimal conditions for low-temperature GOD enzyme production of strain 31.Under the optimal fermentation conditions,the low-temperature GOD enzyme activity reached to 503.22U/mL.Compared with 435.37 U/mL before the optimization,the GOD enzyme activity increased by 15.58%.

关 键 词：海洋 担子菌 葡萄糖氧化酶 响应面法 

分 类 号：Q939.99[生物学—微生物学] TQ925.4[轻工技术与工程—发酵工程]