对阴沟肠杆菌有强抑制活性乳酸菌的分离及鉴定  

Isolation and Identification of Lactic Acid Bacteria with Strong Inhibitory Effects Against Enterobacter cloacae

作　　者：李亚飞 罗春雨 刘奇 Li Yafei;Luo Chunyu;Liu Qi(Pre-clinical College,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学基础医学院,云南大理671000

出　　处：《大理大学学报》2021年第10期32-36,共5页Journal of Dali University

基　　金：大理大学创新团队项目(ZKLX2019105)。

摘　　要：目的:从大理地区泡菜中分离筛选出对阴沟肠杆菌具有抑制优势的乳酸菌菌种,丰富传统乳酸发酵食品潜在的减肥功能。方法:在大理地区收集泡菜样本,用BBL琼脂培养基进行培养分离,通过革兰氏染色、聚合酶链式反应(PCR)鉴定、抑菌活性分析,经条带切胶回收、大量扩增后,构建了TA克隆并进行测序。将获得的基因序列信息用NCBI中的Blast工具进行信息比对,寻找包含该条条带信息的细菌。结果:在18份样本中,分离得到了抑制活性最好的候选菌株,经测序结果比对,明确为植物乳杆菌。结论:成功分离出1株对阴沟肠杆菌标准株ATCC 700323具有较高抑制活性的植物乳酸菌菌种。Objective:To isolate and screen out lactic acid bacteria strains with inhibitory advantages against Enterobacter cloacae from pickles in Dali,and to enrich the potential weight loss function of traditional lactic acid fermented foods.Methods:Pickle samples were collected in Dali area,cultured and separated by BBL agar medium,Gram staining,PCR identification,and antibacterial activity analysis;strips were cut gel,mass amplified,TA clones constructed,and sequenced.The obtained gene sequence information was compared with the Blast data in NCBI to find the bacteria containing this band of information.Results:Among the 18 samples,the candidate strain with the best inhibitory effect was isolated.Through comparing the sequencing results,it was clear that it was Lactobacillus plantarum.Conclusion:A strain of lactic acid bacteria with high inhibitory effect on the standard strain of Enterobacter cloacae ATCC 700323 was successfully isolated.

关 键 词：泡菜 乳酸菌分离 阴沟肠杆菌抑制 菌落PCR 

分 类 号：TS201.3[轻工技术与工程—食品科学]