激活蛋白-1家族JunB、c-Jun、c-Maf、c-Fos在多发性骨髓瘤中的表达  

Expression of Activator Protein-1 Family JunB,c-Jun,c-Maf,c-Fos in Multiple Myeloma

作　　者：燕法红 邱志远[1] 李乾鹏 张晓婷[1] 刘洋[1] 王宝宏[1] YAN Fa-hong;QIU Zhi-yuan;LI Qian-peng;ZHANG Xiao-ting;LIU Yang;WANG Bao-hong(Department of Hematology,the First Affiliated Hospital of Weifang Medical University/Weifang People’s Hospital,Weifang,261041,Shandong,China)

机构地区：[1]潍坊医学院第一附属医院/潍坊市人民医院血液内科,山东潍坊261041

出　　处：《医学信息》2021年第23期44-46,共3页Journal of Medical Information

基　　金：山东省医药卫生科技发展计划项目(编号:2017WS415)。

摘　　要：目的观察转录因子激活蛋白-1(AP-1)家族成员JunB、c-Jun、c-Maf、c-Fos在初诊多发性骨髓瘤(MM)中的表达情况。方法选取我院2019年11月-2020年12月收治的初诊MM患者26例作为MM组,另选同期14例骨髓无恶性细胞的患者作为对照组,应用实时荧光定量PCR(RT-PCR)法检测两组骨髓JunB、c-Jun、c-Maf、c-Fos mRNA的表达水平,比较其差异。结果MM组JunB mRNA表达水平高于对照组[(0.0845±0.0416)vs(0.0538±0.0181)],差异有统计学意义(P<0.05);MM组c-Jun mRNA表达水平高于对照组[(0.0018±0.0011)vs(0.0005±0.0002)],差异有统计学意义(P<0.05);两组c-Maf mRNA、c-Fos mRNA表达水平比较,差异无统计学意义(P>0.05)。结论JunB、c-Jun在多发性骨髓瘤患者中表达上调,可能在其发病中起到一定作用,有望成为新的治疗靶点。Objective To evaluate the expression levels of activator protein-1(AP-1)transcription factor member JunB,c-Jun,c-Maf and c-Fos in newly diagnosed multiple myeloma(MM).Methods A total of 26 newly diagnosed MM patients admitted to our hospital from November 2019 to December 2020 were selected as the MM group,and 14 patients with no malignant cells in bone marrow during the same period were selected as the control group.The expression levels of JunB,c-Jun,c-Maf and c-Fos mRNA in bone marrow of the two groups were detected by real-time fluorescence quantitative PCR(RT-PCR),and the differences were compared.Results The JunB mRNA expression level in the MM group was higher than that in the control group[(0.0845±0.0416)vs(0.0538±0.0181)],and the difference was statistically significant(P<0.05).The expression level of c-Jun mRNA in the MM group was higher than that in the control group[(0.0018±0.0011)vs(0.0005±0.0012)],and the difference was statistically significant(P<0.05).There was no significant difference in the expression levels of c-Maf mRNA and c-Fos mRNA between the two groups(P>0.05).Conclusion The expression of JunB and c-Jun is up-regulated in patients with multiple myeloma,which may play a role in its pathogenesis,and is expected to become a new therapeutic target.

关 键 词：激活蛋白-1 JUNB C-JUN C-MAF C-FOS 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]