小米提取物及其消化液的抗氧化和降糖作用  

Antioxidant and Hypoglycemic Effects of Millet Extract and Its Digestive Solution

作　　者：尹瑞旸 李星 胡锦荣[1] 胡小松[1] 沈群[1] Yin Ruiyang;Li Xing;Hu Jinrong;Hu Xiaosong;Shen Qun(College of Food Science and Nutritional Engineering,China Agricultural University,Key Laboratory of Plant Protein and Grain Processing of Beijing,National Engineering Research Center for Fruits and Vegetables Processing,Beijing 100083;National Food Quality and Safety and Inspection Center,Beijing 100094)

机构地区：[1]中国农业大学食品科学与营养工程学院植物蛋白与谷物加工北京市重点实验室国家果蔬加工工程技术研究中心,北京100083 [2]国家食品质量安全监督检验中心,北京100094

出　　处：《中国食品学报》2021年第11期14-21,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家谷子糜子产业技术体系项目(CARS-07-13.5)。

摘　　要：选用水、乙醇、正丁醇和正己烷为提取剂,测定小米不同溶剂提取物、提取物消化液及沉淀物消化液的DPPH·清除能力、总抗氧化能力,检测该类物质对高脂诱导的HepG2胰岛素抵抗细胞(IR-HepG2)糖吸收的影响。结果表明:水提物及乙醇提取物有较高的抗氧化活性,经体外模拟消化后其抗氧化能力降低(总抗氧化能力分别降低50.00%和54.55%)。与抗氧化结果不同,水提物及乙醇提取物(20μg/mL和40μg/mL)对IR-HepG2胰岛素抵抗改善作用不显著,而正丁醇提取物(40μg/mL和80μg/mL)及正己烷提取物(20,40,80μg/mL)均对IR-HepG2胰岛素抵抗有显著地改善作用。经消化,乙醇提取物对胰岛素抵抗的改善作用显著增强,正丁醇和正己烷提取物对胰岛素抵抗的改善作用无明显变化。正己烷沉淀物消化液、正丁醇沉淀物消化液、水沉淀物消化液及小米消化液均可提高IR-HepG2的糖吸收,而乙醇沉淀物对细胞的胰岛素抵抗并无改善作用,说明小米中起到降糖作用的活性物质主要集中在乙醇提取物中。In order to explore the anti-oxidation and hypoglycemic activity of millet,the DPPH·scavenging capacity and total antioxidant capacity of four different extracts,extracts digestive solution and sediment digestive solution of millet were determined.The glucose uptake levels on PA-induced insulin-resistant HepG2 cells(IR-HepG2)were detected.The results showed that the water extract and ethanol extract had higher antioxidant activity,and their antioxidant capacity decreased after simulated digestion in vitro(the total antioxidant capacity decreased by 50.00%and 54.55%,respectively).Unlike the antioxidant results,the water extract and the ethanol extract(20,40μg/mL)had no significant effect on the improvement of IR-HepG2 insulin resistance.The n-butanol extract(40,80μg/mL)and n-hexane extract(20,40,80μg/mL)all significantly increased the glucose uptake of IR-HepG2.After digestion,the ethanol extract significantly improved insulin resistance,but n-butanol and n-hexane extracts had no effect on insulin resistance.The glucose uptake was enhanced by n-hexane sediment digestive solution,n-butanol sediment digestive solution,water sediment digestive solution and millet digestive solution.However,ethanol sediment digestive solution did not improve insulin resistance,indicating that the hypoglycemic effect of millet was mainly concentrated in ethanol extract.

关 键 词：小米 抗氧化 降糖 体外消化 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]