产脂肪酶蜡状芽孢杆菌菌株分离和鉴定  

Isolation and Identification of a Lipase Producing Strain of Bacillus Cereus

作　　者：刘小玉[1] 付登强[1] LIU Xiaoyu;FU Dengqiang(Coconut Research Institute of Chinese Academy of Tropical Agricultural Sciences/Hainan Engineering Research Center of Arecanut Industry,Wenchang 571339,Hainan)

机构地区：[1]中国热带农业科学院椰子研究所/海南省槟榔产业工程研究中心,海南文昌571339

出　　处：《中国热带农业》2021年第6期68-73,共6页China Tropical Agriculture

基　　金：海南省自然科学基金项目(320QN354);海南省重大科技项目(ZDKJ201817);槟榔产业技术创新团队(1630152017013)。

摘　　要：为筛选出产脂肪酶的菌株,从油茶枯饼发酵液中取样,用中性红油脂培养基进行初筛,改进铜皂—分光光度法测定酶活力进行复筛,获得1株产脂肪酶活性较强的菌株ck-01。结合形态学观察、生理生化试验、16S rDNA序列鉴定和系统发育分析等方法将其鉴定为蜡状芽孢杆菌。对菌株ck-01的酶学性质的研究结果表明,该菌株所产脂肪酶在35℃和pH值7.0的条件下,酶活力为3.35U/mL,Ca^(2+)、Mg^(2+)对酶活有促进作用,Fe^(2+)、Zn^(2+)对酶活有抑制作用,EDTA和SDS可使酶失活。In order to screen the lipase producing strain,the high lipase activity producing strains were isolated from fermented liquid of oil camellia cake,through conducting primary screen using neutral red oil medium and re-screening by determining the enzyme activity using the improved copper soap-spectropho-tometer method.The highest lipase activity producing strain,named ck-01,was further analyzed.Strain ck-01 was identified as bacillus cereus by morphological observation,physiological and biochemical experiment,16S rDNA sequence analysis and phylogenetic analysis.In addition,the enzymatic properties analysis of Strain ck-01 showed that the lipase activity was 3.35 U/mL under the condition of 35℃ and pH 7.0.The lipase activity of Strain ck-01 was promoted by Ca^(2+)and Mg^(2+),but it was inhibited by Fe^(2+)and Zn^(2+).It appeared inactivation after being treated by EDTA and SDS.

关 键 词：脂肪酶 蜡状芽孢杆菌 分离 鉴定 酶学性质 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]