Resveratrol induces proliferation in preosteoblast cell MC3T3-E1 via GATA-1 activating autophagy  

作　　者：Xiang Liu Jun Tao Yueyi Yao Ping Yang Juhui Wang Mali Yu Jianhong Hou Ying Zhang Li Gui 

机构地区：[1]Science and Technology Achievement Incubation Center,Kunming Medical University,Kunming 650500,China [2]Department of Orthopaedics,The Third People’s Hospital of Yunnan Province,Kunming 650101,China [3]Faculty of Nursing,Kunming Medical University,Kunming 650500,China [4]Department of Endocrinology,The Third People’s Hospital of Yunnan Province,Kunming 650101,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第11期1495-1504,共10页生物化学与生物物理学报（英文版）

基　　金：This work was supported in part by the grants from the National Natural Science Foundation of China(Nos.82160175 and 81460173);the Science and Technology Department of Yunnan Province(Nos.2017FF117(-062)and 2019FE001(-023)).

摘　　要：Resveratrol (RSV) could promote osteogenic activity, but its clinical application has been hampered in view of its poor bioavailability. Therefore, it is desirable to identify with certainty the molecular target of its bone mass boosting function, which is crucial to the design of an effective therapeutic strategy for the optimal treatment of osteoporosis. Emerging evidence has indicated that GATA-1, an important transcription factor in megakaryocyte and erythrocyte differentiation, can directly activate autophagy in erythrocytes, alluding to its impact on bone metabolism. In light of this, we sought to determine whether GATA-1 would be a putative target by which RSV would act on osteoblast proliferation and, if so, to explore the underlying mechanism involved in the process. We examined the cell viability, colony formation, cell cyclin expression, autophagy level, and the expression levels of GATA-1 and adenosine 5'-monophosphate {AMP}-activated protein kinase α {AMPKα} in osteoblastic cell strain MC3T3-E1. The results showed that RSV promoted the proliferation process in MC3T3-E1 coupled with increased expression of GATA-1.

关 键 词：GATA-1 RESVERATROL AMPKα PROLIFERATION AUTOPHAGY 

分 类 号：R68[医药卫生—骨科学]